tRNA (cytosine(72)-C(5))-methyltransferase NSUN6 (EC 2.1.1.-) (NOL1/NOP2/Sun and PUA domain-containing protein 1) (NOL1/NOP2/Sun domain family member 6)
1_MSIFP 6_ KISLR 11_ PEVEN 16_ YLKEG 21_ FMNKE 26_ IVTAL 31_ GKQEA 36_ ERKFE 41_ TLLKH 46_ LSHPP 51_ SFTTV 56_ RVNTH 61_ LASVQ 66_ HVKNL 71_ LLDEL 76_ QKQFN 81_ GLSVP 86_ ILQHP 91_ DLQDV 96_ LLIPV 101_ IGPRK 106_ NIKKQ 111_ QCEAI 116_ VGAQC 121_ GNAVL 126_ RGAHV 131_ YAPGI 136_ VSASQ 141_ FMKAG 146_ DVISV 151_ YSDIK 156_ GKCKK 161_ GAKEF 166_ DGTKV 171_ FLGNG 176_ ISELS 181_ RKEIF 186_ SGLPE 191_ LKGMG 196_ IRMTE 201_ PVYLS 206_ PSFDS 211_ VLPRY 216_ LFLQN 221_ LPSAL 226_ VSHVL 231_ NPQPG 236_ EKILD 241_ LCAAP 246_ GGKTT 251_ HIAAL 256_ MHDQG 261_ EVIAL 266_ DKIFN 271_ KVEKI 276_ KQNAL 281_ LLGLN 286_ SIRAF 291_ CFDGT 296_ KAVKL 301_ DMVED 306_ TEGEP 311_ PFLPE 316_ SFDRI 321_ LLDAP 326_ CSGMG 331_ QRPNM 336_ ACTWS 341_ VKEVA 346_ SYQPL 351_ QRKLF 356_ TAAVQ 361_ LLKPE 366_ GVLVY 371_ STCTI 376_ TLAEN 381_ EEQVA 386_ WALTK 391_ FPCLQ 396_ LQPQE 401_ PQIGG 406_ EGMRG 411_ AGLSC 416_ EQLKQ 421_ LQRFD 426_ PSAVP 431_ LPDTD 436_ MDSLR 441_ EARRE 446_ DMLRL 451_ ANKDS 456_ IGFFI 461_AKFVK
1: S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA) (PubMed:26160102, PubMed:27703015, PubMed:28531330). In vitro also methylates tRNA(Thr)(AGT) (PubMed:26160102, PubMed:27703015). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates (PubMed:26160102, PubMed:27703015)