Pseudouridylate synthase TRUB1 (EC 5.4.99.-) (TruB pseudouridine synthase homolog 1) (tRNA pseudouridine 55 synthase TRUB1) (Psi55 synthase TRUB1) (EC 5.4.99.25)
1_MAASE 6_ AAVVS 11_ SPSLK 16_ TDTSP 21_ VLETA 26_ GTVAA 31_ MAATP 36_ SARAA 41_ AAVVA 46_ AAART 51_ GSEAR 56_ VSKAA 61_ LATKL 66_ LSLSG 71_ VFAVH 76_ KPKGP 81_ TSAEL 86_ LNRLK 91_ EKLLA 96_ EAGMP 101_ SPEWT 106_ KRKKQ 111_ TLKIG 116_ HGGTL 121_ DSAAR 126_ GVLVV 131_ GIGSG 136_ TKMLT 141_ SMLSG 146_ SKRYT 151_ AIGEL 156_ GKATD 161_ TLDST 166_ GRVTE 171_ EKPYD 176_ KITQE 181_ DIEGI 186_ LQKFT 191_ GNIMQ 196_ VPPLY 201_ SALKK 206_ DGQRL 211_ STLMK 216_ RGEVV 221_ EAKPA 226_ RPVTV 231_ YSISL 236_ QKFQP 241_ PFFTL 246_ DVECG 251_ GGFYI 256_ RSLVS 261_ DIGKE 266_ LSSCA 271_ NVLEL 276_ TRTKQ 281_ GPFTL 286_ EEHAL 291_ PEDKW 296_ TIDDI 301_ AQSLE 306_ HCSSL 311_ FPAEL 316_ ALKKS 321_ KPESN 326_ EQVLS 331_ CEYIT 336_ LNEPK 341_REDDV
1: Pseudouridine synthase that catalyzes pseudouridylation of mRNAs and tRNAs (PubMed:28073919, PubMed:31477916, PubMed:32926445). Mediates pseudouridylation of mRNAs with the consensus sequence 5'-GUUCNANNC-3', harboring a stem-loop structure (PubMed:28073919, PubMed:31477916). Constitutes the major pseudouridine synthase acting on mRNAs (PubMed:28073919). Also catalyzes pseudouridylation of some tRNAs, including synthesis of pseudouridine(55) from uracil-55, in the psi GC loop of a subset of tRNAs (PubMed:32926445, PubMed:33023933). Promotes the processing of pri-let-7 microRNAs (pri-miRNAs) independently of its RNA pseudouridylate synthase activity (PubMed:32926445). Acts by binding to the stem-loop structure on pri-let-7, preventing LIN28-binding (LIN28A and/or LIN28B), thereby enhancing the interaction between pri-let-7 and the microprocessor DGCR8, which mediates miRNA maturation (PubMed:32926445)