Microprocessor complex subunit DGCR8 (DiGeorge syndrome critical region 8)
1_METDE 6_ SPSPL 11_ PCGPA 16_ GEAVM 21_ ESRAR 26_ PFQAL 31_ PREQS 36_ PPPPL 41_ QTSSG 46_ AEVMD 51_ VGSGG 56_ DGQSE 61_ LPAED 66_ PFNFY 71_ GASLL 76_ SKGSF 81_ SKGRL 86_ LIDPN 91_ CSGHS 96_ PRTAR 101_ HAPAV 106_ RKFSP 111_ DLKLL 116_ KDVKI 121_ SVSFT 126_ ESCRS 131_ KDRKV 136_ LYTGA 141_ ERDVR 146_ AECGL 151_ LLSPV 156_ SGDVH 161_ ACPFG 166_ GSVGD 171_ GVGIG 176_ GESAD 181_ KKDEE 186_ NELDQ 191_ EKRVE 196_ YAVLD 201_ ELEDF 206_ TDNLE 211_ LDEEG 216_ AGGFT 221_ AKAIV 226_ QRDRV 231_ DEEAL 236_ NFPYE 241_ DDFDN 246_ DVDAL 251_ LEEGL 256_ CAPKK 261_ RRTEE 266_ KYGGD 271_ SDHPS 276_ DGETS 281_ VQPMM 286_ TKIKT 291_ VLKSR 296_ GRPPT 301_ EPLPD 306_ GWIMT 311_ FHNSG 316_ VPVYL 321_ HRESR 326_ VVTWS 331_ RPYFL 336_ GTGSI 341_ RKHDP 346_ PLSSI 351_ PCLHY 356_ KKMKD 361_ NEERE 366_ QSSDL 371_ TPSGD 376_ VSPVK 381_ PLSRS 386_ AELEF 391_ PLDEP 396_ DSMGA 401_ DPGPP 406_ DEKDP 411_ LGAEA 416_ APGAL 421_ GQVKA 426_ KVEVC 431_ KDESV 436_ DLEEF 441_ RSYLE 446_ KRFDF 451_ EQVTV 456_ KKFRT 461_ WAERR 466_ QFNRE 471_ MKRKQ 476_ AESER 481_ PILPA 486_ NQKLI 491_ TLSVQ 496_ DAPTK 501_ KEFVI 506_ NPNGK 511_ SEVCI 516_ LHEYM 521_ QRVLK 526_ VRPVY 531_ NFFEC 536_ ENPSE 541_ PFGAS 546_ VTIDG 551_ VTYGS 556_ GTASS 561_ KKLAK 566_ NKAAR 571_ ATLEI 576_ LIPDF 581_ VKQTS 586_ EEKPK 591_ DSEEL 596_ EYFNH 601_ ISIED 606_ SRVYE 611_ LTSKA 616_ GLLSP 621_ YQILH 626_ ECLKR 631_ NHGMG 636_ DTSIK 641_ FEVVP 646_ GKNQK 651_ SEYVM 656_ ACGKH 661_ TVRGW 666_ CKNKR 671_ VGKQL 676_ ASQKI 681_ LQLLH 686_ PHVKN 691_ WGSLL 696_ RMYGR 701_ ESSKM 706_ VKQET 711_ SDKSV 716_ IELQQ 721_ YAKKN 726_ KPNLH 731_ ILSKL 736_ QEEMK 741_ RLAEE 746_ REETR 751_ KKPKM 756_ SIVAS 761_ AQPGG 766_EPLCT
1: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity). Also plays a role in DNA repair by promoting the recruitment of RNF168 to RNF8 and MDC1 at DNA double-strand breaks and subsequently the clearance of DNA breaks (PubMed:34188037)