(3R)-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.n12) (17-beta-hydroxysteroid dehydrogenase 8) (17-beta-HSD 8) (HSD17B8) (3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit) (KAR alpha subunit) (3-oxoacyl-[acyl-carrier-protein] reductase) (Estradiol 17-beta-dehydrogenase 8) (EC 1.1.1.62) (Protein Ke6) (Ke6) (Short chain dehydrogenase/reductase family 30C member 1) (Testosterone 17-beta-dehydrogenase 8) (EC 1.1.1.239)
1_MASQL 6_ QNRLR 11_ SALAL 16_ VTGAG 21_ SGIGR 26_ AVSVR 31_ LAGEG 36_ ATVAA 41_ CDLDR 46_ AAAQE 51_ TVRLL 56_ GGPGS 61_ KEGPP 66_ RGNHA 71_ AFQAD 76_ VSEAR 81_ AARCL 86_ LEQVQ 91_ ACFSR 96_ PPSVV 101_ VSCAG 106_ ITQDE 111_ FLLHM 116_ SEDDW 121_ DKVIA 126_ VNLKG 131_ TFLVT 136_ QAAAQ 141_ ALVSN 146_ GCRGS 151_ IINIS 156_ SIVGK 161_ VGNVG 166_ QTNYA 171_ ASKAG 176_ VIGLT 181_ QTAAR 186_ ELGRH 191_ GIRCN 196_ SVLPG 201_ FIATP 206_ MTQKV 211_ PQKVV 216_ DKITE 221_ MIPMG 226_ HLGDP 231_ EDVAD 236_ VVAFL 241_ ASEDS 246_ GYITG 251_ TSVEV 256_TGGLF
1: Required for the solubility and assembly of the heterotetramer 3-ketoacyl-[acyl carrier protein] (ACP) reductase functional complex (KAR or KAR1) that forms part of the mitochondrial fatty acid synthase (mtFAS). Alpha-subunit of the KAR complex that acts as a scaffold protein required for the stability of carbonyl reductase type-4 (CBR4, beta-subunit of the KAR complex) and for its 3-ketoacyl-ACP reductase activity, thereby participating in mitochondrial fatty acid biosynthesis. Catalyzes the NAD-dependent conversion of (3R)-3-hydroxyacyl-CoA into 3-ketoacyl-CoA (3-oxoacyl-CoA) with no chain length preference; this enzymatic activity is not needed for the KAR function (PubMed:19571038, PubMed:25203508, PubMed:30508570). Prefers (3R)-3-hydroxyacyl-CoA over (3S)-3-hydroxyacyl-CoA and displays enzymatic activity only in the presence of NAD(+) (PubMed:19571038). Cooperates with enoyl-CoA hydratase 1 in mitochondria, together they constitute an alternative route to the auxiliary enzyme pathways for the breakdown of Z-PUFA (cis polyunsaturated fatty acid) enoyl-esters (Probable) (PubMed:30508570). NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol (17beta-estradiol or E2). Has very low activity towards testosterone and dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one). Primarily an oxidative enzyme, it can switch to a reductive mode determined in the appropriate physiologic milieu and catalyze the reduction of estrone (E1) to form biologically active 17beta-estradiol (PubMed:17978863)