RNA polymerase-associated protein RTF1 homolog
1_MRGRL 6_ CVGRA 11_ AAAAA 16_ AVAVP 21_ LAGGQ 26_ EGSPG 31_ GGRRG 36_ SRGTT 41_ MVKKR 46_ KGRVV 51_ IDSDT 56_ EDSGS 61_ DENLD 66_ QELLS 71_ LAKRK 76_ RSDSE 81_ EKEPP 86_ VSQPA 91_ ASSDS 96_ ETSDS 101_ DDEWT 106_ FGSNK 111_ NKKKG 116_ KARKI 121_ EKKGT 126_ MKKQA 131_ NKTAS 136_ SGSSD 141_ KDSSA 146_ ESSAP 151_ EEGEV 156_ SDSDS 161_ NSSSS 166_ SSDSD 171_ SSSED 176_ EEFHD 181_ GYGED 186_ LMGDE 191_ EDRAR 196_ LEQMT 201_ EKERE 206_ QELFN 211_ RIEKR 216_ EVLKR 221_ RFEIK 226_ KKLKT 231_ AKKKE 236_ KKEKK 241_ KKQEE 246_ EQEKK 251_ KLTQI 256_ QESQV 261_ TSHNK 266_ ERRSK 271_ RDEKL 276_ DKKSQ 281_ AMEEL 286_ KAERE 291_ KRKNR 296_ TAELL 301_ AKKQP 306_ LKTSE 311_ VYSDD 316_ EEEEE 321_ DDKSS 326_ EKSDR 331_ SSRTS 336_ SSDEE 341_ EEKEE 346_ IPPKS 351_ QPVSL 356_ PEELN 361_ RVRLS 366_ RHKLE 371_ RWCHM 376_ PFFAK 381_ TVTGC 386_ FVRIG 391_ IGNHN 396_ SKPVY 401_ RVAEI 406_ TGVVE 411_ TAKVY 416_ QLGGT 421_ RTNKG 426_ LQLRH 431_ GNDQR 436_ VFRLE 441_ FVSNQ 446_ EFTES 451_ EFMKW 456_ KEAMF 461_ SAGMQ 466_ LPTLD 471_ EINKK 476_ ELSIK 481_ EALNY 486_ KFNDQ 491_ DIEEI 496_ VKEKE 501_ RFRKA 506_ PPNYA 511_ MKKTQ 516_ LLKEK 521_ AMAED 526_ LGDQD 531_ KAKQI 536_ QDQLN 541_ ELEER 546_ AEALD 551_ RQRTK 556_ NISAI 561_ SYINQ 566_ RNREW 571_ NIVES 576_ EKALV 581_ AESHN 586_ MKNQQ 591_ MDPFT 596_ RRQCK 601_ PTIVS 606_ NSRDP 611_ AVQAA 616_ ILAQL 621_ NAKYG 626_ SGVLP 631_ DAPKE 636_ MSKGQ 641_ GKDKD 646_ LNSKS 651_ ASDLS 656_ EDLFK 661_ VHDFD 666_ VKIDL 671_ QVPSS 676_ ESKAL 681_ AITSK 686_ APPAK 691_ DGAPR 696_ RSLNL 701_EDYKK
1: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex (By similarity)