Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (EC 2.4.1.258) (Asparagine-linked glycosylation protein 3 homolog) (Dol-P-Man-dependent alpha(1-3)-mannosyltransferase) (Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase) (Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase) (Not56-like protein)
1_MAAGL 6_ RKRGR 11_ SGSAA 16_ QAEGL 21_ CKQWL 26_ QRAWQ 31_ ERRLL 36_ LREPR 41_ YTLLV 46_ AACLC 51_ LAEVG 56_ ITFWV 61_ IHRVA 66_ YTEID 71_ WKAYM 76_ AEVEG 81_ VINGT 86_ YDYTQ 91_ LQGDT 96_ GPLVY 101_ PAGFV 106_ YIFMG 111_ LYYAT 116_ SRGTD 121_ IRMAQ 126_ NIFAV 131_ LYLAT 136_ LLLVF 141_ LIYHQ 146_ TCKVP 151_ PFVFF 156_ FMCCA 161_ SYRVH 166_ SIFVL 171_ RLFND 176_ PVAMV 181_ LLFLS 186_ INLLL 191_ AQRWG 196_ WGCCF 201_ FSLAV 206_ SVKMN 211_ VLLFA 216_ PGLLF 221_ LLLTQ 226_ FGFRG 231_ ALPKL 236_ GICAG 241_ LQVVL 246_ GLPFL 251_ LENPS 256_ GYLSR 261_ SFDLG 266_ RQFLF 271_ HWTVN 276_ WRFLP 281_ EALFL 286_ HRAFH 291_ LALLT 296_ AHLTL 301_ LLLFA 306_ LCRWH 311_ RTGES 316_ ILSLL 321_ RDPSK 326_ RKVPP 331_ QPLTP 336_ NQIVS 341_ TLFTS 346_ NFIGI 351_ CFSRS 356_ LHYQF 361_ YVWYF 366_ HTLPY 371_ LLWAM 376_ PARWL 381_ THLLR 386_ LLVLG 391_ LIELS 396_ WNTYP 401_ STSCS 406_ SAALH 411_ ICHAV 416_ ILLQL 421_ WLGPQ 426_ PFPKS 431_TQHSK
1: Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first dolichyl beta-D-mannosyl phosphate derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-dolichol to produce Man(6)GlcNAc(2)-PP-dolichol (PubMed:10581255). Man(6)GlcNAc(2)-PP-dolichol is a substrate for ALG9, the following enzyme in the biosynthetic pathway (PubMed:10581255)