Regulator of nonsense transcripts 1 (EC 3.6.4.12) (EC 3.6.4.13) (ATP-dependent helicase RENT1) (Nonsense mRNA reducing factor 1) (NORF1) (Up-frameshift suppressor 1 homolog) (hUpf1)
1_MSVEA 6_ YGPSS 11_ QTLTF 16_ LDTEE 21_ AELLG 26_ ADTQG 31_ SEFEF 36_ TDFTL 41_ PSQTQ 46_ TPPGG 51_ PGGPG 56_ GGGAG 61_ GPGGA 66_ GAGAA 71_ AGQLD 76_ AQVGP 81_ EGILQ 86_ NGAVD 91_ DSVAK 96_ TSQLL 101_ AELNF 106_ EEDEE 111_ DTYYT 116_ KDLPI 121_ HACSY 126_ CGIHD 131_ PACVV 136_ YCNTS 141_ KKWFC 146_ NGRGN 151_ TSGSH 156_ IVNHL 161_ VRAKC 166_ KEVTL 171_ HKDGP 176_ LGETV 181_ LECYN 186_ CGCRN 191_ VFLLG 196_ FIPAK 201_ ADSVV 206_ VLLCR 211_ QPCAS 216_ QSSLK 221_ DINWD 226_ SSQWQ 231_ PLIQD 236_ RCFLS 241_ WLVKI 246_ PSEQE 251_ QLRAR 256_ QITAQ 261_ QINKL 266_ EELWK 271_ ENPSA 276_ TLEDL 281_ EKPGV 286_ DEEPQ 291_ HVLLR 296_ YEDAY 301_ QYQNI 306_ FGPLV 311_ KLEAD 316_ YDKKL 321_ KESQT 326_ QDNIT 331_ VRWDL 336_ GLNKK 341_ RIAYF 346_ TLPKT 351_ DSGNE 356_ DLVII 361_ WLRDM 366_ RLMQG 371_ DEICL 376_ RYKGD 381_ LAPLW 386_ KGIGH 391_ VIKVP 396_ DNYGD 401_ EIAIE 406_ LRSSV 411_ GAPVE 416_ VTHNF 421_ QVDFV 426_ WKSTS 431_ FDRMQ 436_ SALKT 441_ FAVDE 446_ TSVSG 451_ YIYHK 456_ LLGHE 461_ VEDVI 466_ IKCQL 471_ PKRFT 476_ AQGLP 481_ DLNHS 486_ QVYAV 491_ KTVLQ 496_ RPLSL 501_ IQGPP 506_ GTGKT 511_ VTSAT 516_ IVYHL 521_ ARQGN 526_ GPVLV 531_ CAPSN 536_ IAVDQ 541_ LTEKI 546_ HQTGL 551_ KVVRL 556_ CAKSR 561_ EAIDS 566_ PVSFL 571_ ALHNQ 576_ IRNMD 581_ SMPEL 586_ QKLQQ 591_ LKDET 596_ GELSS 601_ ADEKR 606_ YRALK 611_ RTAER 616_ ELLMN 621_ ADVIC 626_ CTCVG 631_ AGDPR 636_ LAKMQ 641_ FRSIL 646_ IDEST 651_ QATEP 656_ ECMVP 661_ VVLGA 666_ KQLIL 671_ VGDHC 676_ QLGPV 681_ VMCKK 686_ AAKAG 691_ LSQSL 696_ FERLV 701_ VLGIR 706_ PIRLQ 711_ VQYRM 716_ HPALS 721_ AFPSN 726_ IFYEG 731_ SLQNG 736_ VTAAD 741_ RVKKG 746_ FDFQW 751_ PQPDK 756_ PMFFY 761_ VTQGQ 766_ EEIAS 771_ SGTSY 776_ LNRTE 781_ AANVE 786_ KITTK 791_ LLKAG 796_ AKPDQ 801_ IGIIT 806_ PYEGQ 811_ RSYLV 816_ QYMQF 821_ SGSLH 826_ TKLYQ 831_ EVEIA 836_ SVDAF 841_ QGREK 846_ DFIIL 851_ SCVRA 856_ NEHQG 861_ IGFLN 866_ DPRRL 871_ NVALT 876_ RARYG 881_ VIIVG 886_ NPKAL 891_ SKQPL 896_ WNHLL 901_ NYYKE 906_ QKVLV 911_ EGPLN 916_ NLRES 921_ LMQFS 926_ KPRKL 931_ VNTIN 936_ PGARF 941_ MTTAM 946_ YDARE 951_ AIIPG 956_ SVYDR 961_ SSQGR 966_ PSSMY 971_ FQTHD 976_ QIGMI 981_ SAGPS 986_ HVAAM 991_ NIPIP 996_ FNLVM 1001_ PPMPP 1006_ PGYFG 1011_ QANGP 1016_ AAGRG 1021_ TPKGK 1026_ TGRGG 1031_ RQKNR 1036_ FGLPG 1041_ PSQTN 1046_ LPNSQ 1051_ ASQDV 1056_ ASQPF 1061_ SQGAL 1066_ TQGYI 1071_ SMSQP 1076_ SQMSQ 1081_ PGLSQ 1086_ PELSQ 1091_ DSYLG 1096_ DEFKS 1101_ QIDVA 1106_ LSQDS 1111_ TYQGE 1116_ RAYQH 1121_GGVTG
1: RNA-dependent helicase required for nonsense-mediated decay (NMD) of aberrant mRNAs containing premature stop codons and modulates the expression level of normal mRNAs (PubMed:11163187, PubMed:16086026, PubMed:18172165, PubMed:21145460, PubMed:21419344, PubMed:24726324). Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD (PubMed:11544179, PubMed:25220460). Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex (PubMed:19417104). In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD (PubMed:21419344). Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors (PubMed:12554878). UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways (PubMed:18447585). Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2 (PubMed:16086026, PubMed:18172165). For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (PubMed:18447585, PubMed:25220460). The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (PubMed:21145460). Together with UPF2 and dependent on TDRD6, mediates the degradation of mRNA harboring long 3'UTR by inducing the NMD machinery (By similarity). Also capable of unwinding double-stranded DNA and translocating on single-stranded DNA (PubMed:30218034)