Ribosomal RNA small subunit methyltransferase NEP1 (EC 2.1.1.-) (18S rRNA (pseudouridine(1248)-N1)-methyltransferase) (18S rRNA Psi1248 methyltransferase) (Nucleolar protein EMG1 homolog) (Protein C2f) (Ribosome biogenesis protein NEP1)
1_MAAPS 6_ DGFKP 11_ RERSG 16_ GEQAQ 21_ DWDAL 26_ PPKRP 31_ RLGAG 36_ NKIGG 41_ RRLIV 46_ VLEGA 51_ SLETV 56_ KVGKT 61_ YELLN 66_ CDKHK 71_ SILLK 76_ NGRDP 81_ GEARP 86_ DITHQ 91_ SLLML 96_ MDSPL 101_ NRAGL 106_ LQVYI 111_ HTQKN 116_ VLIEV 121_ NPQTR 126_ IPRTF 131_ DRFCG 136_ LMVQL 141_ LHKLS 146_ VRAAD 151_ GPQKL 156_ LKVIK 161_ NPVSD 166_ HFPVG 171_ CMKVG 176_ TSFSI 181_ PVVSD 186_ VRELV 191_ PSSDP 196_ IVFVV 201_ GAFAH 206_ GKVSV 211_ EYTEK 216_ MVSIS 221_ NYPLS 226_ AALTC 231_ AKLTT 236_AFEEV
1: S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed:20047967). Also has an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes (By similarity). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797)