Polyribonucleotide 5'-hydroxyl-kinase Clp1 (EC 2.7.1.78) (Polyadenylation factor Clp1) (Polynucleotide kinase Clp1) (Pre-mRNA cleavage complex II protein Clp1)
1_MGEEA 6_ NDDKK 11_ PTTKF 16_ ELERE 21_ TELRF 26_ EVEAS 31_ QSVQL 36_ ELLTG 41_ MAEIF 46_ GTELT 51_ RNKKF 56_ TFDAG 61_ AKVAV 66_ FTWHG 71_ CSVQL 76_ SGRTE 81_ VAYVS 86_ KDTPM 91_ LLYLN 96_ THTAL 101_ EQMRR 106_ QAEKE 111_ EERGP 116_ RVMVV 121_ GPTDV 126_ GKSTV 131_ CRLLL 136_ NYAVR 141_ LGRRP 146_ TYVEL 151_ DVGQG 156_ SVSIP 161_ GTMGA 166_ LYIER 171_ PADVE 176_ EGFSI 181_ QAPLV 186_ YHFGS 191_ TTPGT 196_ NIKLY 201_ NKITS 206_ RLADV 211_ FNQRC 216_ EVNRR 221_ ASVSG 226_ CVINT 231_ CGWVK 236_ GSGYQ 241_ ALVHA 246_ ASAFE 251_ VDVVV 256_ VLDQE 261_ RLYNE 266_ LKRDL 271_ PHFVR 276_ TVLLP 281_ KSGGV 286_ VERSK 291_ DFRRE 296_ CRDER 301_ IREYF 306_ YGFRG 311_ CFYPH 316_ AFNVK 321_ FSDVK 326_ IYKVG 331_ APTIP 336_ DSCLP 341_ LGMSQ 346_ EDNQL 351_ KLVPV 356_ TPGRD 361_ MVHHL 366_ LSVST 371_ AEGTE 376_ ENLSE 381_ TSVAG 386_ FIVVT 391_ SVDLE 396_ HQVFT 401_ VLSPA 406_ PRPLP 411_ KNFLL 416_IMDIR
1: Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:24766809, PubMed:24766810). Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809, PubMed:24766810). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing