Riboflavin kinase (EC 2.7.1.26) (ATP:riboflavin 5'-phosphotransferase) (Flavokinase)
1_MRHLP 6_ YFCRG 11_ QVVRG 16_ FGRGS 21_ KQLGI 26_ PTANF 31_ PEQVV 36_ DNLPA 41_ DISTG 46_ IYYGW 51_ ASVGS 56_ GDVHK 61_ MVVSI 66_ GWNPY 71_ YKNTK 76_ KSMET 81_ HIMHT 86_ FKEDF 91_ YGEIL 96_ NVAIV 101_ GYLRP 106_ EKNFD 111_ SLESL 116_ ISAIQ 121_ GDIEE 126_ AKKRL 131_ ELPEH 136_ LKIKE 141_ DNFFQ 146_VSKSK
1: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase