NEDD8-conjugating enzyme UBE2F (EC 2.3.2.32) (NEDD8 carrier protein UBE2F) (NEDD8 protein ligase UBE2F) (NEDD8-conjugating enzyme 2) (RING-type E3 NEDD8 transferase UBE2F) (Ubiquitin-conjugating enzyme E2 F)
1_MLTLA 6_ SKLKR 11_ DDGLK 16_ GSRTA 21_ ATASD 26_ STRRV 31_ SVRDK 36_ LLVKE 41_ VAELE 46_ ANLPC 51_ TCKVH 56_ FPDPN 61_ KLHCF 66_ QLTVT 71_ PDEGY 76_ YQGGK 81_ FQFET 86_ EVPDA 91_ YNMVP 96_ PKVKC 101_ LTKIW 106_ HPNIT 111_ ETGEI 116_ CLSLL 121_ REHSI 126_ DGTGW 131_ APTRT 136_ LKDVV 141_ WGLNS 146_ LFTDL 151_ LNFDD 156_ PLNIE 161_ AAEHH 166_ LRDKE 171_ DFRNK 176_VDDYI
1: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins (PubMed:19250909, PubMed:23201271). Together with the E3 ubiquitin ligase RNF7/RBX2, specifically neddylates cullin-5 (CUL5) (PubMed:19250909, PubMed:23201271, PubMed:23300442). Does not neddylate CUL1, CUL2, CUL3, CUL4A or CUL4B (PubMed:19250909, PubMed:23201271). Mediates neddylation of the CUL9-RBX1 complex (PubMed:38605244)
2: (Microbial infection) Following infection by HIV-1 virus, participates to HIV-1 Vif protein-mediated ubiquitination and degradation of APOBEC3G by mediating neddylation of cullin-5 (CUL5)