DNA topoisomerase I, mitochondrial (TOP1mt) (EC 5.6.2.1)
1_MRVVR 6_ LLRLR 11_ AALTL 16_ LGEVP 21_ RRPAS 26_ RGVPG 31_ SRRTQ 36_ KGSGA 41_ RWEKE 46_ KHEDG 51_ VKWRQ 56_ LEHKG 61_ PYFAP 66_ PYEPL 71_ PDGVR 76_ FFYEG 81_ RPVRL 86_ SVAAE 91_ EVATF 96_ YGRML 101_ DHEYT 106_ TKEVF 111_ RKNFF 116_ NDWRK 121_ EMAVE 126_ EREVI 131_ KSLDK 136_ CDFTE 141_ IHRYF 146_ VDKAA 151_ ARKVL 156_ SREEK 161_ QKLKE 166_ EAEKL 171_ QQEFG 176_ YCILD 181_ GHQEK 186_ IGNFK 191_ IEPPG 196_ LFRGR 201_ GDHPK 206_ MGMLK 211_ RRITP 216_ EDVVI 221_ NCSRD 226_ SKIPE 231_ PPAGH 236_ QWKEV 241_ RSDNT 246_ VTWLA 251_ AWTES 256_ VQNSI 261_ KYIML 266_ NPCSK 271_ LKGET 276_ AWQKF 281_ ETARR 286_ LRGFV 291_ DEIRS 296_ QYRAD 301_ WKSRE 306_ MKTRQ 311_ RAVAL 316_ YFIDK 321_ LALRA 326_ GNEKE 331_ DGEAA 336_ DTVGC 341_ CSLRV 346_ EHVQL 351_ HPEAD 356_ GCQHV 361_ VEFDF 366_ LGKDC 371_ IRYYN 376_ RVPVE 381_ KPVYK 386_ NLQLF 391_ MENKD 396_ PRDDL 401_ FDRLT 406_ TTSLN 411_ KHLQE 416_ LMDGL 421_ TAKVF 426_ RTYNA 431_ SITLQ 436_ EQLRA 441_ LTRAE 446_ DSIAA 451_ KILSY 456_ NRANR 461_ VVAIL 466_ CNHQR 471_ ATPST 476_ FEKSM 481_ QNLQT 486_ KIQAK 491_ KEQVA 496_ EARAE 501_ LRRAR 506_ AEHKA 511_ QGDGK 516_ SRSVL 521_ EKKRR 526_ LLEKL 531_ QEQLA 536_ QLSVQ 541_ ATDKE 546_ ENKQV 551_ ALGTS 556_ KLNYL 561_ DPRIS 566_ IAWCK 571_ RFRVP 576_ VEKIY 581_ SKTQR 586_ ERFAW 591_ ALAMA 596_GEDFE
1: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity)