Protein N-terminal asparagine amidohydrolase (EC 3.5.1.121) (Protein NH2-terminal asparagine amidohydrolase) (PNAA) (Protein NH2-terminal asparagine deamidase) (PNAD) (Protein N-terminal Asn amidase) (Protein N-terminal asparagine amidase) (Protein NTN-amidase)
1_MPLLV 6_ EGRRV 11_ RLPQS 16_ AGDLV 21_ RAHPP 26_ LEERA 31_ RLLRG 36_ QSVQQ 41_ VGPQG 46_ LLYVQ 51_ QRELA 56_ VTSPK 61_ DGSIS 66_ ILGSD 71_ DATTC 76_ HIVVL 81_ RHTGN 86_ GATCL 91_ THCDG 96_ TDTKA 101_ EVPLI 106_ MNSIK 111_ SFSDH 116_ AQCGR 121_ LEVHL 126_ VGGFS 131_ DDRQL 136_ SQKLT 141_ HQLLS 146_ EFDRQ 151_ EDDIH 156_ LVTLC 161_ VTELN 166_ DREEN 171_ ENHFP 176_ VIYGI 181_ AVNIK 186_ TAEIY 191_ RASFQ 196_ DRGPE 201_ EQLRA 206_ ARTLA 211_ GGPMI 216_ SIYDA 221_ ETEQL 226_ RIGPY 231_ SWTPF 236_ PHVDF 241_ WLHQD 246_ DKQIL 251_ ENLST 256_ SPLAE 261_ PPHFV 266_ EHIRS 271_ TLMFL 276_ KKHPS 281_ PAHTL 286_ FSGNK 291_ ALLYK 296_ KNEDG 301_LWEKI
1: N-terminal asparagine deamidase that mediates deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by NTAN1/PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N-terminal peptidyl Asn