Crossover junction endonuclease EME1 (EC 3.1.22.-) (MMS4 homolog) (hMMS4)
1_MALKK 6_ SSPSL 11_ DSGDS 16_ DSEEL 21_ PTFAF 26_ LKKEP 31_ SSTKR 36_ RQPER 41_ EEKIV 46_ VVDIS 51_ DCEAS 56_ CPPAP 61_ ELFSP 66_ PVPEI 71_ AETVT 76_ QTQPV 81_ RLLSS 86_ ESEDE 91_ EEFIP 96_ LAQRL 101_ TCKFL 106_ THKQL 111_ SPEDS 116_ SSPVK 121_ SVLDH 126_ QNNEG 131_ ASCDW 136_ KKPFP 141_ KIPEV 146_ PLHDT 151_ PERSA 156_ ADNKD 161_ LILDP 166_ CCQLP 171_ AYLST 176_ CPGQS 181_ SSLAV 186_ TKTNS 191_ DILPP 196_ QKKTK 201_ PSQKV 206_ QGRGS 211_ HGCRQ 216_ QRQAR 221_ QKEST 226_ LRRQE 231_ RKNAA 236_ LVTRM 241_ KAQRP 246_ EECLK 251_ HIIVV 256_ LDPVL 261_ LQMEG 266_ GGQLL 271_ GALQT 276_ MECRC 281_ VIEAQ 286_ AVPCS 291_ VTWRR 296_ RAGPS 301_ EDRED 306_ WVEEP 311_ TVLVL 316_ LRAEA 321_ FVSMI 326_ DNGKQ 331_ GSLDS 336_ TMKGK 341_ ETLQG 346_ FVTDI 351_ TAKTA 356_ GKALS 361_ LVIVD 366_ QEKCF 371_ SAQNP 376_ PRRGK 381_ QGANK 386_ QTKKQ 391_ QQRQP 396_ EASIG 401_ SMVSR 406_ VDAEE 411_ ALVDL 416_ QLHTE 421_ AQAQI 426_ VQSWK 431_ ELADF 436_ TCAFT 441_ KAVAE 446_ APFKK 451_ LRDET 456_ TFSFC 461_ LESDW 466_ AGGVK 471_ VDLAG 476_ RGLAL 481_ VWRRQ 486_ IQQLN 491_ RVSLE 496_ MASAV 501_ VNAYP 506_ SPQLL 511_ VQAYQ 516_ QCFSD 521_ KERQN 526_ LLADI 531_ QVRRG 536_ EGVTS 541_ TSRRI 546_ GPELS 551_ RRIYL 556_ QMTTL 561_QPHLS
1: Non-catalytic subunit of the structure-specific, heterodimeric DNA endonuclease MUS81-EME1 which is involved in the maintenance of genome stability. In the complex, EME1 is required for DNA cleavage, participating in DNA recognition and bending (PubMed:12686547, PubMed:12721304, PubMed:14617801, PubMed:17289582, PubMed:24733841, PubMed:24813886, PubMed:35290797, PubMed:39015284). MUS81-EME1 cleaves 3'-flaps and nicked Holliday junctions, and exhibit limited endonuclease activity with 5' flaps and nicked double-stranded DNAs (PubMed:24733841, PubMed:35290797). Active during prometaphase, MUS81-EME1 resolves mitotic recombination intermediates, including Holliday junctions, which form during homologous recombination (PubMed:14617801, PubMed:24813886)