5-methylcytosine rRNA methyltransferase NSUN4 (EC 2.1.1.-) (5-methylcytosine tRNA methyltransferase NSUN4) (EC 2.1.1.-) (NOL1/NOP2/Sun domain family member 4)
1_MAALT 6_ LRGVR 11_ ELLKR 16_ VDLAT 21_ VPRRH 26_ RYKKK 31_ WAATE 36_ PKFPA 41_ VRLAL 46_ QNFDM 51_ TYSVQ 56_ FGDLW 61_ PSIRV 66_ SLLSE 71_ QKYGA 76_ LVNNF 81_ AAWDH 86_ VSAKL 91_ EQLSA 96_ KDFVN 101_ EAISH 106_ WELQS 111_ EGGQS 116_ AAPSP 121_ ASWAC 126_ SPNLR 131_ CFTFD 136_ RGDIS 141_ RFPPA 146_ RPGSL 151_ GVMEY 156_ YLMDA 161_ ASLLP 166_ VLALG 171_ LQPGD 176_ IVLDL 181_ CAAPG 186_ GKTLA 191_ LLQTG 196_ CCRNL 201_ AANDL 206_ SPSRI 211_ ARLQK 216_ ILHSY 221_ VPEEI 226_ RDGNQ 231_ VRVTS 236_ WDGRK 241_ WGELE 246_ GDTYD 251_ RVLVD 256_ VPCTT 261_ DRHSL 266_ HEEEN 271_ NIFKR 276_ SRKKE 281_ RQILP 286_ VLQVQ 291_ LLAAG 296_ LLATK 301_ PGGHV 306_ VYSTC 311_ SLSHL 316_ QNEYV 321_ VQGAI 326_ ELLAN 331_ QYSIQ 336_ VQVED 341_ LTHFR 346_ RVFMD 351_ TFCFF 356_ SSCQV 361_ GELVI 366_ PNLMA 371_ NFGPM 376_YFCKM
1: Mitochondrial RNA cytosine C(5)-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as rRNAs, mRNAs and some long non-coding RNAs (lncRNAs) (PubMed:21531335, PubMed:23022348, PubMed:39019044). Involved in mitochondrial ribosome small subunit (SSU) maturation by catalyzing methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU) (PubMed:21531335, PubMed:23022348). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled (PubMed:21531335, PubMed:23022348). In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4 (PubMed:23022348). Also acts as a regulator of innate immunity by marking double-stranded mitochondrial RNAs(mt-dsRNAs) generated in response to stress: catalyzes m5C modification on mitochondrial RNAs, such as a mRNAs and lncRNAs, with a preference for the termini of light-strand lncRNAs, promoting their degradation and cytosolic release (PubMed:39019044). Modified light-strand lncRNAs are then recognized by C1QBP reader and recruited to the mitochondrial degradosome complex, which promotes their degradation (PubMed:39019044)