Protein N-terminal glutamine amidohydrolase (EC 3.5.1.122) (Protein NH2-terminal glutamine deamidase) (N-terminal Gln amidase) (Nt(Q)-amidase) (WDYHV motif-containing protein 1)
1_MEGNG 6_ PAAVH 11_ YQPAS 16_ PPRDA 21_ CVYSS 26_ CYCEE 31_ NIWKL 36_ CEYIK 41_ NHDQY 46_ PLEEC 51_ YAVFI 56_ SNERK 61_ MIPIW 66_ KQQAR 71_ PGDGP 76_ VIWDY 81_ HVVLL 86_ HVSSG 91_ GQNFI 96_ YDLDT 101_ VLPFP 106_ CLFDT 111_ YVEDA 116_ FKSDD 121_ DIHPQ 126_ FRRKF 131_ RVIRA 136_ DSYLK 141_ NFASD 146_ RSHMK 151_ DSSGN 156_ WREPP 161_ PPYPC 166_ IETGD 171_ SKMNL 176_ NDFIS 181_ MDPKV 186_ GWGAV 191_ YTLSE 196_FTHRF
1: Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine