Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (PNGase) (hPNGase) (EC 3.5.1.52) (N-glycanase 1) (Peptide:N-glycanase)
1_MAAAA 6_ LGSSS 11_ GSASP 16_ AVAEL 21_ CQNTP 26_ ETFLE 31_ ASKLL 36_ LTYAD 41_ NILRN 46_ PNDEK 51_ YRSIR 56_ IGNTA 61_ FSTRL 66_ LPVRG 71_ AVECL 76_ FEMGF 81_ EEGET 86_ HLIFP 91_ KKASV 96_ EQLQK 101_ IRDLI 106_ AIERS 111_ SRLDG 116_ SNKSH 121_ KVKSS 126_ QQPAA 131_ STQLP 136_ TTPSS 141_ NPSGL 146_ NQHTR 151_ NRQGQ 156_ SSDPP 161_ SASTV 166_ AADSA 171_ ILEVL 176_ QSNIQ 181_ HVLVY 186_ ENPAL 191_ QEKAL 196_ ACIPV 201_ QELKR 206_ KSQEK 211_ LSRAR 216_ KLDKG 221_ INISD 226_ EDFLL 231_ LELLH 236_ WFKEE 241_ FFHWV 246_ NNVLC 251_ SKCGG 256_ QTRSR 261_ DRSLL 266_ PSDDE 271_ LKWGA 276_ KEVED 281_ HYCDA 286_ CQFSN 291_ RFPRY 296_ NNPEK 301_ LLETR 306_ CGRCG 311_ EWANC 316_ FTLCC 321_ RAVGF 326_ EARYV 331_ WDYTD 336_ HVWTE 341_ VYSPS 346_ QQRWL 351_ HCDAC 356_ EDVCD 361_ KPLLY 366_ EIGWG 371_ KKLSY 376_ VIAFS 381_ KDEVV 386_ DVTWR 391_ YSCKH 396_ EEVIA 401_ RRTKV 406_ KEALL 411_ RDTIN 416_ GLNKQ 421_ RQLFL 426_ SENRR 431_ KELLQ 436_ RIIVE 441_ LVEFI 446_ SPKTP 451_ KPGEL 456_ GGRIS 461_ GSVAW 466_ RVARG 471_ EMGLQ 476_ RKETL 481_ FIPCE 486_ NEKIS 491_ KQLHL 496_ CYNIV 501_ KDRYV 506_ RVSNN 511_ NQTIS 516_ GWENG 521_ VWKME 526_ SIFRK 531_ VETDW 536_ HMVYL 541_ ARKEG 546_ SSFAY 551_ ISWKF 556_ ECGSV 561_ GLKVD 566_ SISIR 571_ TSSQT 576_ FQTGT 581_ VEWKL 586_ RSDTA 591_ QVELT 596_ GDNSL 601_ HSYAD 606_ FSGAT 611_ EVILE 616_ AELSR 621_ GDGDV 626_ AWQHT 631_ QLFRQ 636_ SLNDH 641_ EENCL 646_EIIIK
1: Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins