CDK5 regulatory subunit-associated protein 3 (CDK5 activator-binding protein C53) (LXXLL/leucine-zipper-containing ARF-binding protein) (Protein HSF-27)
1_MEDHQ 6_ HVPID 11_ IQTSK 16_ LLDWL 21_ VDRRH 26_ CSLKW 31_ QSLVL 36_ TIREK 41_ INAAI 46_ QDMPE 51_ SEEIA 56_ QLLSG 61_ SYIHY 66_ FHCLR 71_ ILDLL 76_ KGTEA 81_ STKNI 86_ FGRYS 91_ SQRMK 96_ DWQEI 101_ IALYE 106_ KDNTY 111_ LVELS 116_ SLLVR 121_ NVNYE 126_ IPSLK 131_ KQIAK 136_ CQQLQ 141_ QEYSR 146_ KEEEC 151_ QAGAA 156_ EMREQ 161_ FYHSC 166_ KQYGI 171_ TGENV 176_ RGELL 181_ ALVKD 186_ LPSQL 191_ AEIGA 196_ AAQQS 201_ LGEAI 206_ DVYQA 211_ SVGFV 216_ CESPT 221_ EQVLP 226_ MLRFV 231_ QKRGN 236_ STVYE 241_ WRTGT 246_ EPSVV 251_ ERPHL 256_ EELPE 261_ QVAED 266_ AIDWG 271_ DFGVE 276_ AVSEG 281_ TDSGI 286_ SAEAA 291_ GIDWG 296_ IFPES 301_ DSKDP 306_ GGDGI 311_ DWGDD 316_ AVALQ 321_ ITVLE 326_ AGTQA 331_ PEGVA 336_ RGPDA 341_ LTLLE 346_ YTETR 351_ NQFLD 356_ ELMEL 361_ EIFLA 366_ QRAVE 371_ LSEEA 376_ DVLSV 381_ SQFQL 386_ APAIL 391_ QGQTK 396_ EKMVT 401_ MVSVL 406_ EDLIG 411_ KLTSL 416_ QLQHL 421_ FMILA 426_ SPRYV 431_ DRVTE 436_ FLQQK 441_ LKQSQ 446_ LLALK 451_ KELMV 456_ QKQQE 461_ ALEEQ 466_ AALEP 471_ KLDLL 476_ LEKTK 481_ ELQKL 486_ IEADI 491_ SKRYS 496_ GRPVN 501_LMGTS
1: Substrate adapter of E3 ligase complexes mediating ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, and which is involved in various processes, such as ribosome recycling and reticulophagy (also called ER-phagy) (PubMed:23152784, PubMed:30635284, PubMed:32851973, PubMed:36121123, PubMed:36543799, PubMed:37595036, PubMed:38383785, PubMed:38383789). As part of the UREL complex, plays a key role in ribosome recycling by promoting mono-ufmylation of RPL26/uL24 subunit of the 60S ribosome (PubMed:38383785, PubMed:38383789). Ufmylation of RPL26/uL24 occurs on free 60S ribosomes following ribosome dissociation: it weakens the junction between post-termination 60S subunits and SEC61 translocons, promoting release and recycling of the large ribosomal subunit from the endoplasmic reticulum membrane (PubMed:38383785, PubMed:38383789). Ufmylation of RPL26/uL24 and subsequent 60S ribosome recycling either take place after normal termination of translation or after ribosome stalling during cotranslational translocation at the endoplasmic reticulum (PubMed:32851973, PubMed:37595036, PubMed:38383785, PubMed:38383789). Within the UREL complex, CDK5RAP3 acts as a substrate adapter that constrains UFL1 ligase activity to mono-ufmylate RPL26/uL24 at 'Lys-134' (PubMed:36121123, PubMed:38383785, PubMed:38383789). The UREL complex is also involved in reticulophagy in response to endoplasmic reticulum stress by promoting ufmylation of proteins such as CYB5R3, thereby promoting lysosomal degradation of ufmylated proteins (PubMed:36543799). Also acts as a regulator of transcription: negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation (PubMed:17785205, PubMed:20228063). Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint (PubMed:15790566, PubMed:19223857). Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation (PubMed:16173922). May also play a role in the rupture of the nuclear envelope during apoptosis (PubMed:23478299). May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1 (PubMed:21283629). Required for liver development (By similarity)
2: (Microbial infection) May be negatively regulated by hepatitis B virus large envelope protein mutant pre-s2 to promote mitotic entry