Legumain (EC 3.4.22.34) (Asparaginyl endopeptidase) (Protease, cysteine 1)
1_MVWKV 6_ AVFLS 11_ VALGI 16_ GAVPI 21_ DDPED 26_ GGKHW 31_ VVIVA 36_ GSNGW 41_ YNYRH 46_ QADAC 51_ HAYQI 56_ IHRNG 61_ IPDEQ 66_ IVVMM 71_ YDDIA 76_ YSEDN 81_ PTPGI 86_ VINRP 91_ NGTDV 96_ YQGVP 101_ KDYTG 106_ EDVTP 111_ QNFLA 116_ VLRGD 121_ AEAVK 126_ GIGSG 131_ KVLKS 136_ GPQDH 141_ VFIYF 146_ TDHGS 151_ TGILV 156_ FPNED 161_ LHVKD 166_ LNETI 171_ HYMYK 176_ HKMYR 181_ KMVFY 186_ IEACE 191_ SGSMM 196_ NHLPD 201_ NINVY 206_ ATTAA 211_ NPRES 216_ SYACY 221_ YDEKR 226_ STYLG 231_ DWYSV 236_ NWMED 241_ SDVED 246_ LTKET 251_ LHKQY 256_ HLVKS 261_ HTNTS 266_ HVMQY 271_ GNKTI 276_ STMKV 281_ MQFQG 286_ MKRKA 291_ SSPVP 296_ LPPVT 301_ HLDLT 306_ PSPDV 311_ PLTIM 316_ KRKLM 321_ NTNDL 326_ EESRQ 331_ LTEEI 336_ QRHLD 341_ ARHLI 346_ EKSVR 351_ KIVSL 356_ LAASE 361_ AEVEQ 366_ LLSER 371_ APLTG 376_ HSCYP 381_ EALLH 386_ FRTHC 391_ FNWHS 396_ PTYEY 401_ ALRHL 406_ YVLVN 411_ LCEKP 416_ YPLHR 421_ IKLSM 426_DHVCL
1: Has a strict specificity for hydrolysis of asparaginyl bonds (PubMed:23776206). Can also cleave aspartyl bonds slowly, especially under acidic conditions (PubMed:23776206). Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (PubMed:9872320). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol (By similarity). Required for normal lysosomal protein degradation in renal proximal tubules (By similarity). Required for normal degradation of internalized EGFR (By similarity). Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity)