DNA endonuclease RBBP8 (EC 3.1.-.-) (CtBP-interacting protein) (CtIP) (Retinoblastoma-binding protein 8) (RBBP-8) (Retinoblastoma-interacting protein and myosin-like) (RIM) (Sporulation in the absence of SPO11 protein 2 homolog) (SAE2)
1_MNISG 6_ SSCGS 11_ PNSAD 16_ TSSDF 21_ KDLWT 26_ KLKEC 31_ HDREV 36_ QGLQV 41_ KVTKL 46_ KQERI 51_ LDAQR 56_ LEEFF 61_ TKNQQ 66_ LREQQ 71_ KVLHE 76_ TIKVL 81_ EDRLR 86_ AGLCD 91_ RCAVT 96_ EEHMR 101_ KKQQE 106_ FENIR 111_ QQNLK 116_ LITEL 121_ MNERN 126_ TLQEE 131_ NKKLS 136_ EQLQQ 141_ KIEND 146_ QQHQA 151_ AELEC 156_ EEDVI 161_ PDSPI 166_ TAFSF 171_ SGVNR 176_ LRRKE 181_ NPHVR 186_ YIEQT 191_ HTKLE 196_ HSVCA 201_ NEMRK 206_ VSKSS 211_ THPQH 216_ NPNEN 221_ EILVA 226_ DTYDQ 231_ SQSPM 236_ AKAHG 241_ TSSYT 246_ PDKSS 251_ FNLAT 256_ VVAET 261_ LGLGV 266_ QEESE 271_ TQGPM 276_ SPLGD 281_ ELYHC 286_ LEGNH 291_ KKQPF 296_ EESTR 301_ NTEDS 306_ LRFSD 311_ STSKT 316_ PPQEE 321_ LPTRV 326_ SSPVF 331_ GATSS 336_ IKSGL 341_ DLNTS 346_ LSPSL 351_ LQPGK 356_ KKHLK 361_ TLPFS 366_ NTCIS 371_ RLEKT 376_ RSKSE 381_ DSALF 386_ THHSL 391_ GSEVN 396_ KIIIQ 401_ SSNKQ 406_ ILINK 411_ NISES 416_ LGEQN 421_ RTEYG 426_ KDSNT 431_ DKHLE 436_ PLKSL 441_ GGRTS 446_ KRKKT 451_ EEESE 456_ HEVSC 461_ PQASF 466_ DKENA 471_ FPFPM 476_ DNQFS 481_ MNGDC 486_ VMDKP 491_ LDLSD 496_ RFSAI 501_ QRQEK 506_ SQGSE 511_ TSKNK 516_ FRQVT 521_ LYEAL 526_ KTIPK 531_ GFSSS 536_ RKASD 541_ GNCTL 546_ PKDSP 551_ GEPCS 556_ QECII 561_ LQPLN 566_ KCSPD 571_ NKPSL 576_ QIKEE 581_ NAVFK 586_ IPLRP 591_ RESLE 596_ TENVL 601_ DDIKS 606_ AGSHE 611_ PIKIQ 616_ TRSDH 621_ GGCEL 626_ ASVLQ 631_ LNPCR 636_ TGKIK 641_ SLQNN 646_ QDVSF 651_ ENIQW 656_ SIDPG 661_ ADLSQ 666_ YKMDV 671_ TVIDT 676_ KDGSQ 681_ SKLGG 686_ ETVDM 691_ DCTLV 696_ SETVL 701_ LKMKK 706_ QEQKG 711_ EKSSN 716_ EERKM 721_ NDSLE 726_ DMFDR 731_ TTHEE 736_ YESCL 741_ ADSFS 746_ QAADE 751_ EEELS 756_ TATKK 761_ LHTHG 766_ DKQDK 771_ VKQKA 776_ FVEPY 781_ FKGDE 786_ RETSL 791_ QNFPH 796_ IEVVR 801_ KKEER 806_ RKLLG 811_ HTCKE 816_ CEIYY 821_ ADMPA 826_ EEREK 831_ KLASC 836_ SRHRF 841_ RYIPP 846_ NTPEN 851_ FWEVG 856_ FPSTQ 861_ TCMER 866_ GYIKE 871_ DLDPC 876_ PRPKR 881_ RQPYN 886_ AIFSP 891_KGKEQ
1: Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway (PubMed:17965729, PubMed:19202191, PubMed:19759395, PubMed:20064462, PubMed:23273981, PubMed:26721387, PubMed:27814491, PubMed:27889449, PubMed:30787182). HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse (PubMed:17965729, PubMed:19202191, PubMed:23273981, PubMed:27814491, PubMed:27889449, PubMed:30787182). Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ) (PubMed:19202191). Specifically promotes the endonuclease activity of the MRN complex to clear DNA ends containing protein adducts: recruited to DSBs by NBN following phosphorylation by CDK1, and promotes the endonuclease activity of MRE11 to clear protein-DNA adducts and generate clean double-strand break ends (PubMed:27814491, PubMed:27889449, PubMed:30787182, PubMed:33836577). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA (PubMed:16581787, PubMed:17965729, PubMed:19759395, PubMed:20064462). Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (PubMed:15485915, PubMed:16818604). During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (By similarity). Binds preferentially to DNA Y-junctions and to DNA substrates with blocked ends and promotes intermolecular DNA bridging (PubMed:30601117)