Structure-specific endonuclease subunit SLX1 (EC 3.1.-.-) (GIY-YIG domain-containing protein 1)
1_MGPAG 6_ VAARP 11_ GRFFG 16_ VYLLY 21_ CLNPR 26_ YRGRV 31_ YVGFT 36_ VNTAR 41_ RVQQH 46_ NGGRK 51_ KGGAW 56_ RTSGR 61_ GPWEM 66_ VLVVH 71_ GFPSS 76_ VAALR 81_ FEWAW 86_ QHPHA 91_ SRRLA 96_ HVGPR 101_ LRGET 106_ AFAFH 111_ LRVLA 116_ HMLRA 121_ PPWAR 126_ LPLTL 131_ RWVRP 136_ DLRQD 141_ LCLPP 146_ PPHVP 151_ LAFGP 156_ PPPQA 161_ PAPRR 166_ RAGPF 171_ DDAEP 176_ EPDQG 181_ DPGAC 186_ CSLCA 191_ QTIQD 196_ EEGPL 201_ CCPHP 206_ GCLLR 211_ AHVIC 216_ LAEEF 221_ LQEEP 226_ GQLLP 231_ LEGQC 236_ PCCEK 241_ SLLWG 246_ DLIWL 251_ CQMDT 256_ EKEVE 261_ DSELE 266_EAHWT
1: Catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products