tRNA-splicing endonuclease subunit Sen34 (EC 4.6.1.16) (Leukocyte receptor cluster member 5) (tRNA-intron endonuclease Sen34) (HsSen34)
1_MLVVE 6_ VANGR 11_ SLVWG 16_ AEAVQ 21_ ALRER 26_ LGVGG 31_ RTVGA 36_ LPRGP 41_ RQNSR 46_ LGLPL 51_ LLMPE 56_ EARLL 61_ AEIGA 66_ VTLVS 71_ APRPD 76_ SRHHS 81_ LALTS 86_ FKRQQ 91_ EESFQ 96_ EQSAL 101_ AAEAR 106_ ETRRQ 111_ ELLEK 116_ ITEGQ 121_ AAKKQ 126_ KLEQA 131_ SGASS 136_ SQEAG 141_ SSQAA 146_ KEDET 151_ SDGQA 156_ SGEQE 161_ EAGPS 166_ SSQAG 171_ PSNGV 176_ APLPR 181_ SALLV 186_ QLATA 191_ RPRPV 196_ KARPL 201_ DWRVQ 206_ SKDWP 211_ HAGRP 216_ AHELR 221_ YSIYR 226_ DLWER 231_ GFFLS 236_ AAGKF 241_ GGDFL 246_ VYPGD 251_ PLRFH 256_ AHYIA 261_ QCWAP 266_ EDTIP 271_ LQDLV 276_ AAGRL 281_ GTSVR 286_ KTLLL 291_ CSPQP 296_ DGKVV 301_YTSLQ
1: Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events