Protein adenylyltransferase FICD (EC 2.7.7.n1) (AMPylator FICD) (De-AMPylase FICD) (EC 3.1.4.-) (FIC domain-containing protein) (Huntingtin yeast partner E) (Huntingtin-interacting protein 13) (HIP-13) (Huntingtin-interacting protein E)
1_MMLIP 6_ MASVM 11_ AVTEP 16_ KWVSV 21_ WSRFL 26_ WVTLL 31_ SMVLG 36_ SLLAL 41_ LLPLG 46_ AVEEQ 51_ CLAVL 56_ KGLYL 61_ LRSKP 66_ DRAQH 71_ AATKC 76_ TSPST 81_ ELSIT 86_ SRGAT 91_ LLVAK 96_ TKASP 101_ AGKLE 106_ ARAAL 111_ NQALE 116_ MKRQG 121_ KREKA 126_ QKLFM 131_ HALKM 136_ DPDFV 141_ DALTE 146_ FGIFS 151_ EEDKD 156_ IIQAD 161_ YLYTR 166_ ALTIS 171_ PYHEK 176_ ALVNR 181_ DRTLP 186_ LVEEI 191_ DQRYF 196_ SIIDS 201_ KVKKV 206_ MSIPK 211_ GNSAL 216_ RRVME 221_ ETYYH 226_ HIYHT 231_ VAIEG 236_ NTLTL 241_ SEIRH 246_ ILETR 251_ YAVPG 256_ KSLEE 261_ QNEVI 266_ GMHAA 271_ MKYIN 276_ TTLVS 281_ RIGSV 286_ TISDV 291_ LEIHR 296_ RVLGY 301_ VDPVE 306_ AGRFR 311_ TTQVL 316_ VGHHI 321_ PPHPQ 326_ DVEKQ 331_ MQEFV 336_ QWLNS 341_ EEAMN 346_ LHPVE 351_ FAALA 356_ HYKLV 361_ YIHPF 366_ IDGNG 371_ RTSRL 376_ LMNLI 381_ LMQAG 386_ YPPIT 391_ IRKEQ 396_ RSDYY 401_ HVLEA 406_ ANEGD 411_ VRPFI 416_ RFIAK 421_ CTETT 426_ LDTLL 431_ FATTE 436_ YSVAL 441_ PEAQP 446_ NHSGF 451_KETLP
1: Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity). The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (PubMed:36136088). Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083, PubMed:36136088). In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity). In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity). Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083)