Egl nine homolog 1 (EC 1.14.11.29) (Hypoxia-inducible factor prolyl hydroxylase 2) (HIF-PH2) (HIF-prolyl hydroxylase 2) (HPH-2) (Prolyl hydroxylase domain-containing protein 2) (PHD2) (SM-20)
1_MANDS 6_ GGPGG 11_ PSPSE 16_ RDRQY 21_ CELCG 26_ KMENL 31_ LRCSR 36_ CRSSF 41_ YCCKE 46_ HQRQD 51_ WKKHK 56_ LVCQG 61_ SEGAL 66_ GHGVG 71_ PHQHS 76_ GPAPP 81_ AAVPP 86_ PRAGA 91_ REPRK 96_ AAARR 101_ DNASG 106_ DAAKG 111_ KVKAK 116_ PPADP 121_ AAAAS 126_ PCRAA 131_ AGGQG 136_ SAVAA 141_ EAEPG 146_ KEEPP 151_ ARSSL 156_ FQEKA 161_ NLYPP 166_ SNTPG 171_ DALSP 176_ GGGLR 181_ PNGQT 186_ KPLPA 191_ LKLAL 196_ EYIVP 201_ CMNKH 206_ GICVV 211_ DDFLG 216_ KETGQ 221_ QIGDE 226_ VRALH 231_ DTGKF 236_ TDGQL 241_ VSQKS 246_ DSSKD 251_ IRGDK 256_ ITWIE 261_ GKEPG 266_ CETIG 271_ LLMSS 276_ MDDLI 281_ RHCNG 286_ KLGSY 291_ KINGR 296_ TKAMV 301_ ACYPG 306_ NGTGY 311_ VRHVD 316_ NPNGD 321_ GRCVT 326_ CIYYL 331_ NKDWD 336_ AKVSG 341_ GILRI 346_ FPEGK 351_ AQFAD 356_ IEPKF 361_ DRLLF 366_ FWSDR 371_ RNPHE 376_ VQPAY 381_ ATRYA 386_ ITVWY 391_ FDADE 396_ RARAK 401_ VKYLT 406_ GEKGV 411_ RVELN 416_ KPSDS 421_VGKDV
1: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif