Ubiquitin-like modifier-activating enzyme 5 (Ubiquitin-activating enzyme 5) (ThiFP1) (UFM1-activating enzyme) (Ubiquitin-activating enzyme E1 domain-containing protein 1)
1_MAESV 6_ ERLQQ 11_ RVQEL 16_ ERELA 21_ QERSL 26_ QVPRS 31_ GDGGG 36_ GRVRI 41_ EKMSS 46_ EVVDS 51_ NPYSR 56_ LMALK 61_ RMGIV 66_ SDYEK 71_ IRTFA 76_ VAIVG 81_ VGGVG 86_ SVTAE 91_ MLTRC 96_ GIGKL 101_ LLFDY 106_ DKVEL 111_ ANMNR 116_ LFFQP 121_ HQAGL 126_ SKVQA 131_ AEHTL 136_ RNINP 141_ DVLFE 146_ VHNYN 151_ ITTVE 156_ NFQHF 161_ MDRIS 166_ NGGLE 171_ EGKPV 176_ DLVLS 181_ CVDNF 186_ EARMT 191_ INTAC 196_ NELGQ 201_ TWMES 206_ GVSEN 211_ AVSGH 216_ IQLII 221_ PGESA 226_ CFACA 231_ PPLVV 236_ AANID 241_ EKTLK 246_ REGVC 251_ AASLP 256_ TTMGV 261_ VAGIL 266_ VQNVL 271_ KFLLN 276_ FGTVS 281_ FYLGY 286_ NAMQD 291_ FFPTM 296_ SMKPN 301_ PQCDD 306_ RNCRK 311_ QQEEY 316_ KKKVA 321_ ALPKQ 326_ EVIQE 331_ EEEII 336_ HEDNE 341_ WGIEL 346_ VSEVS 351_ EEELK 356_ NFSGP 361_ VPDLP 366_ EGITV 371_ AYTIP 376_ KKQED 381_ SVTEL 386_ TVEDS 391_ GESLE 396_DLMAK
1: E1-like enzyme which specifically catalyzes the first step in ufmylation (PubMed:15071506, PubMed:18442052, PubMed:20368332, PubMed:25219498, PubMed:26929408, PubMed:27545674, PubMed:27545681, PubMed:27653677, PubMed:30412706, PubMed:30626644, PubMed:34588452). Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP (PubMed:20368332, PubMed:26929408, PubMed:27653677, PubMed:30412706). Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer (PubMed:27653677). Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding (PubMed:29295865). Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism (PubMed:27653677, PubMed:34588452). Ufmylation plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (PubMed:30412706, PubMed:32160526, PubMed:35394863). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes (By similarity)