ADP/ATP translocase 4 (ADP,ATP carrier protein 4) (Adenine nucleotide translocator 4) (ANT 4) (Solute carrier family 25 member 31) (Sperm flagellar energy carrier protein)
1_MHREP 6_ AKKKA 11_ EKRLF 16_ DASSF 21_ GKDLL 26_ AGGVA 31_ AAVSK 36_ TAVAP 41_ IERVK 46_ LLLQV 51_ QASSK 56_ QISPE 61_ ARYKG 66_ MVDCL 71_ VRIPR 76_ EQGFF 81_ SFWRG 86_ NLANV 91_ IRYFP 96_ TQALN 101_ FAFKD 106_ KYKQL 111_ FMSGV 116_ NKEKQ 121_ FWRWF 126_ LANLA 131_ SGGAA 136_ GATSL 141_ CVVYP 146_ LDFAR 151_ TRLGV 156_ DIGKG 161_ PEERQ 166_ FKGLG 171_ DCIMK 176_ IAKSD 181_ GIAGL 186_ YQGFG 191_ VSVQG 196_ IIVYR 201_ ASYFG 206_ AYDTV 211_ KGLLP 216_ KPKKT 221_ PFLVS 226_ FFIAQ 231_ VVTTC 236_ SGILS 241_ YPFDT 246_ VRRRM 251_ MMQSG 256_ EAKRQ 261_ YKGTL 266_ DCFVK 271_ IYQHE 276_ GISSF 281_ FRGAF 286_ SNVLR 291_ GTGGA 296_ LVLVL 301_ YDKIK 306_EFFHI
1: ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell (By similarity) (PubMed:15670820). Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane (By similarity). Specifically required during spermatogenesis, probably to mediate ADP:ATP exchange in spermatocytes (PubMed:17137571). Large ATP supplies from mitochondria may be critical for normal progression of spermatogenesis during early stages of meiotic prophase I, including DNA double-strand break repair and chromosomal synapsis (By similarity). In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity (By similarity). Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis (By similarity). Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A31/ANT4 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity) (By similarity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it (By similarity). Among nucleotides, may also exchange ADP for dATP and dADP (PubMed:15670820). Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death (By similarity). It is however unclear if SLC25A31/ANT4 constitutes a pore-forming component of mPTP or regulates it (By similarity)