GDP-fucose protein O-fucosyltransferase 1 (EC 2.4.1.221) (Peptide-O-fucosyltransferase 1) (O-FucT-1)
1_MGAAA 6_ WARPL 11_ SVSFL 16_ LLLLP 21_ LPGMP 26_ AGSWD 31_ PAGYL 36_ LYCPC 41_ MGRFG 46_ NQADH 51_ FLGSL 56_ AFAKL 61_ LNRTL 66_ AVPPW 71_ IEYQH 76_ HKPPF 81_ TNLHV 86_ SYQKY 91_ FKLEP 96_ LQAYH 101_ RVISL 106_ EDFME 111_ KLAPT 116_ HWPPE 121_ KRVAY 126_ CFEVA 131_ AQRSP 136_ DKKTC 141_ PMKEG 146_ NPFGP 151_ FWDQF 156_ HVSFN 161_ KSELF 166_ TGISF 171_ SASYR 176_ EQWSQ 181_ RFSPK 186_ EHPVL 191_ ALPGA 196_ PAQFP 201_ VLEEH 206_ RPLQK 211_ YMVWS 216_ DEMVK 221_ TGEAQ 226_ IHAHL 231_ VRPYV 236_ GIHLR 241_ IGSDW 246_ KNACA 251_ MLKDG 256_ TAGSH 261_ FMASP 266_ QCVGY 271_ SRSTA 276_ APLTM 281_ TMCLP 286_ DLKEI 291_ QRAVK 296_ LWVRS 301_ LDAQS 306_ VYVAT 311_ DSESY 316_ VPELQ 321_ QLFKG 326_ KVKVV 331_ SLKPE 336_ VAQVD 341_ LYILG 346_ QADHF 351_ IGNCV 356_ SSFTA 361_ FVKRE 366_ RDLQG 371_ RPSSF 376_ FGMDR 381_PPKLR
1: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs)