Alpha-1,3/1,6-mannosyltransferase ALG2 (EC 2.4.1.132) (EC 2.4.1.257) (Asparagine-linked glycosylation protein 2 homolog) (GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase) (GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase) (GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase)
1_MAEEQ 6_ GRERD 11_ SVPKP 16_ SVLFL 21_ HPDLG 26_ VGGAE 31_ RLVLD 36_ AALAL 41_ QARGC 46_ SVKIW 51_ TAHYD 56_ PGHCF 61_ AESRE 66_ LPVRC 71_ AGDWL 76_ PRGLG 81_ WGGRG 86_ AAVCA 91_ YVRMV 96_ FLALY 101_ VLFLA 106_ DEEFD 111_ VVVCD 116_ QVSAC 121_ IPVFR 126_ LARRR 131_ KKILF 136_ YCHFP 141_ DLLLT 146_ KRDSF 151_ LKRLY 156_ RAPID 161_ WIEEY 166_ TTGMA 171_ DCILV 176_ NSQFT 181_ AAVFK 186_ ETFKS 191_ LSHID 196_ PDVLY 201_ PSLNV 206_ TSFDS 211_ VVPEK 216_ LDDLV 221_ PKGKK 226_ FLLLS 231_ INRYE 236_ RKKNL 241_ TLALE 246_ ALVQL 251_ RGRLT 256_ SQDWE 261_ RVHLI 266_ VAGGY 271_ DERVL 276_ ENVEH 281_ YQELK 286_ KMVQQ 291_ SDLGQ 296_ YVTFL 301_ RSFSD 306_ KQKIS 311_ LLHSC 316_ TCVLY 321_ TPSNE 326_ HFGIV 331_ PLEAM 336_ YMQCP 341_ VIAVN 346_ SGGPL 351_ ESIDH 356_ SVTGF 361_ LCEPD 366_ PVHFS 371_ EAIEK 376_ FIREP 381_ SLKAT 386_ MGLAG 391_ RARVK 396_ EKFSP 401_ EAFTE 406_ QLYRY 411_VTKLL
1: Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the second and third mannose residues to the dolichol-linked oligosaccharide chain, to produce Man3GlcNAc(2)-PP-dolichol core oligosaccharide. Man3GlcNAc(2)-PP-dolichol is a substrate for ALG11, the following enzyme in the biosynthetic pathway (PubMed:12684507, PubMed:35136180). While both alpha 1,3 and alpha 1,6 linkages are possible, the sequential addition of alpha 1,3 followed by alpha 1,6 is probably the preferred route (PubMed:35136180)