Protein O-mannose kinase (POMK) (EC 2.7.1.183) (Protein kinase-like protein SgK196) (Sugen kinase 196)
1_MEKQP 6_ QNSRR 11_ GLAPR 16_ EVPPA 21_ VGLLL 26_ IMALM 31_ NTLLY 36_ LCLDH 41_ FFIAP 46_ RQSTV 51_ DPTHC 56_ PYGHF 61_ RIGQM 66_ KNCSP 71_ WLSCE 76_ ELRTE 81_ VRQLK 86_ RVGEG 91_ AVKRV 96_ FLSEW 101_ KEHKV 106_ ALSQL 111_ TSLEM 116_ KDDFL 121_ HGLQM 126_ LKSLQ 131_ GTHVV 136_ TLLGY 141_ CEDDN 146_ TMLTE 151_ YHPLG 156_ SLSNL 161_ EETLN 166_ LSKYQ 171_ NVNTW 176_ QHRLE 181_ LAMDY 186_ VSIIN 191_ YLHHS 196_ PVGTR 201_ VMCDS 206_ NDLPK 211_ TLSQY 216_ LLTSN 221_ FSILA 226_ NDLDA 231_ LPLVN 236_ HSSGM 241_ LVKCG 246_ HRELH 251_ GDFVA 256_ PEQLW 261_ PYGED 266_ VPFHD 271_ DLMPS 276_ YDEKI 281_ DIWKI 286_ PDISS 291_ FLLGH 296_ IEGSD 301_ MVRFH 306_ LFDIH 311_ KACKS 316_ QTPSE 321_ RPTAQ 326_ DVLET 331_ YQKVL 336_ DTLRD 341_AMMSQ
1: Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif