tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial (EC 2.1.1.-) (Methyltransferase-like protein 8) (mRNA N(3)-methylcytidine methyltransferase METTL8) (EC 2.1.1.-)
1_MNMIW 6_ RNSIS 11_ CLRLG 16_ KVPHR 21_ YQSGY 26_ HPVAP 31_ LGSRI 36_ LTDPA 41_ KVFEH 46_ NMWDH 51_ MQWSK 56_ EEEAA 61_ ARKKV 66_ KENSA 71_ VRVLL 76_ EEQVK 81_ YEREA 86_ SKYWD 91_ TFYKI 96_ HKNKF 101_ FKDRN 106_ WLLRE 111_ FPEIL 116_ PVDQK 121_ PEEKA 126_ RESSW 131_ DHVKT 136_ SATNR 141_ FSRMH 146_ CPTVP 151_ DEKNH 156_ YEKSS 161_ GSSEG 166_ QSKTE 171_ SDFSN 176_ LDSEK 181_ HKKGP 186_ METGL 191_ FPGSN 196_ ATFRI 201_ LEVGC 206_ GAGNS 211_ VFPIL 216_ NTLEN 221_ SPESF 226_ LYCCD 231_ FASGA 236_ VELVK 241_ SHSSY 246_ RATQC 251_ FAFVH 256_ DVCDD 261_ GLPYP 266_ FPDGI 271_ LDVIL 276_ LVFVL 281_ SSIHP 286_DRTLF
1: Mitochondrial S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of mitochondrial tRNA(Ser)(UCN) and tRNA(Thr) (PubMed:34774131, PubMed:35017528). N(3)-methylcytidine methylation modification regulates mitochondrial translation efficiency and is required for activity of the respiratory chain (PubMed:34774131, PubMed:35017528). N(3)-methylcytidine methylation of mitochondrial tRNA(Ser)(UCN) requires the formation of N(6)-dimethylallyladenosine(37) (i6A37) by TRIT1 as prerequisite (PubMed:34774131, PubMed:35017528). May also mediate N(3)-methylcytidine modification of mRNAs (PubMed:28655767). The existence of N(3)-methylcytidine modification on mRNAs is however unclear, and additional evidences are required to confirm the role of the N(3)-methylcytidine-specific mRNA methyltransferase activity of METTL8 in vivo (PubMed:33313824, PubMed:34774131)