E3 ubiquitin-protein ligase SMURF2 (hSMURF2) (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase SMURF2) (SMAD ubiquitination regulatory factor 2) (SMAD-specific E3 ubiquitin-protein ligase 2)
1_MSNPG 6_ GRRNG 11_ PVKLR 16_ LTVLC 21_ AKNLV 26_ KKDFF 31_ RLPDP 36_ FAKVV 41_ VDGSG 46_ QCHST 51_ DTVKN 56_ TLDPK 61_ WNQHY 66_ DLYIG 71_ KSDSV 76_ TISVW 81_ NHKKI 86_ HKKQG 91_ AGFLG 96_ CVRLL 101_ SNAIN 106_ RLKDT 111_ GYQRL 116_ DLCKL 121_ GPNDN 126_ DTVRG 131_ QIVVS 136_ LQSRD 141_ RIGTG 146_ GQVVD 151_ CSRLF 156_ DNDLP 161_ DGWEE 166_ RRTAS 171_ GRIQY 176_ LNHIT 181_ RTTQW 186_ ERPTR 191_ PASEY 196_ SSPGR 201_ PLSCF 206_ VDENT 211_ PISGT 216_ NGATC 221_ GQSSD 226_ PRLAE 231_ RRVRS 236_ QRHRN 241_ YMSRT 246_ HLHTP 251_ PDLPE 256_ GYEQR 261_ TTQQG 266_ QVYFL 271_ HTQTG 276_ VSTWH 281_ DPRVP 286_ RDLSN 291_ INCEE 296_ LGPLP 301_ PGWEI 306_ RNTAT 311_ GRVYF 316_ VDHNN 321_ RTTQF 326_ TDPRL 331_ SANLH 336_ LVLNR 341_ QNQLK 346_ DQQQQ 351_ QVVSL 356_ CPDDT 361_ ECLTV 366_ PRYKR 371_ DLVQK 376_ LKILR 381_ QELSQ 386_ QQPQA 391_ GHCRI 396_ EVSRE 401_ EIFEE 406_ SYRQV 411_ MKMRP 416_ KDLWK 421_ RLMIK 426_ FRGEE 431_ GLDYG 436_ GVARE 441_ WLYLL 446_ SHEML 451_ NPYYG 456_ LFQYS 461_ RDDIY 466_ TLQIN 471_ PDSAV 476_ NPEHL 481_ SYFHF 486_ VGRIM 491_ GMAVF 496_ HGHYI 501_ DGGFT 506_ LPFYK 511_ QLLGK 516_ SITLD 521_ DMELV 526_ DPDLH 531_ NSLVW 536_ ILEND 541_ ITGVL 546_ DHTFC 551_ VEHNA 556_ YGEII 561_ QHELK 566_ PNGKS 571_ IPVNE 576_ ENKKE 581_ YVRLY 586_ VNWRF 591_ LRGIE 596_ AQFLA 601_ LQKGF 606_ NEVIP 611_ QHLLK 616_ TFDEK 621_ ELELI 626_ ICGLG 631_ KIDVN 636_ DWKVN 641_ TRLKH 646_ CTPDS 651_ NIVKW 656_ FWKAV 661_ EFFDE 666_ ERRAR 671_ LLQFV 676_ TGSSR 681_ VPLQG 686_ FKALQ 691_ GAAGP 696_ RLFTI 701_ HQIDA 706_ CTNNL 711_ PKAHT 716_ CFNRI 721_ DIPPY 726_ ESYEK 731_ LYEKL 736_ LTAIE 741_ETCGF
1: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:11016919). Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling (PubMed:11163210, PubMed:12717440, PubMed:21791611). In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1 (PubMed:18448069). Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation (PubMed:11016919, PubMed:11158580, PubMed:11389444). SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation (PubMed:11389444). Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation (PubMed:30696809)
2: (Microbial infection) In case of filoviruses Ebola/EBOV and Marburg/MARV infection, the complex formed by viral matrix protein VP40 and SMURF2 facilitates virus budding