Three-prime repair exonuclease 1 (EC 3.1.11.2) (3'-5' exonuclease TREX1) (Deoxyribonuclease III) (DNase III)
1_MGSQA 6_ LPPGP 11_ MQTLI 16_ FFDME 21_ ATGLP 26_ FSQPK 31_ VTELC 36_ LLAVH 41_ RCALE 46_ SPPTS 51_ QGPPP 56_ TVPPP 61_ PRVVD 66_ KLSLC 71_ VAPGK 76_ ACSPA 81_ ASEIT 86_ GLSTA 91_ VLAAH 96_ GRQCF 101_ DDNLA 106_ NLLLA 111_ FLRRQ 116_ PQPWC 121_ LVAHN 126_ GDRYD 131_ FPLLQ 136_ AELAM 141_ LGLTS 146_ ALDGA 151_ FCVDS 156_ ITALK 161_ ALERA 166_ SSPSE 171_ HGPRK 176_ SYSLG 181_ SIYTR 186_ LYGQS 191_ PPDSH 196_ TAEGD 201_ VLALL 206_ SICQW 211_ RPQAL 216_ LRWVD 221_ AHARP 226_ FGTIR 231_ PMYGV 236_ TASAR 241_ TKPRP 246_ SAVTT 251_ TAHLA 256_ TTRNT 261_ SPSLG 266_ ESRGT 271_ KDLPP 276_ VKDPG 281_ ALSRE 286_ GLLAP 291_ LGLLA 296_ ILTLA 301_ VATLY 306_GLSLA
1: Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini (PubMed:10391904, PubMed:10393201, PubMed:17293595). Prevents cell-intrinsic initiation of autoimmunity (PubMed:10391904, PubMed:10393201, PubMed:17293595). Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements (PubMed:10391904, PubMed:10393201, PubMed:17293595). Plays a key role in degradation of DNA fragments at cytosolic micronuclei arising from genome instability: its association with the endoplasmic reticulum membrane directs TREX1 to ruptured micronuclei, leading to micronuclear DNA degradation (PubMed:33476576). Micronuclear DNA degradation is required to limit CGAS activation and subsequent inflammation (PubMed:33476576). Unless degraded, these DNA fragments accumulate in the cytosol and activate the cGAS-STING innate immune signaling, leading to the production of type I interferon (PubMed:33476576). Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates (PubMed:18045533). Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light (PubMed:23993650). During GZMA-mediated cell death, contributes to DNA damage in concert with NME1 (PubMed:16818237). NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair (PubMed:16818237)