Ubiquitin-like-conjugating enzyme ATG3 (EC 2.3.2.-) (Autophagy-related protein 3) (APG3-like) (hApg3) (Protein PC3-96)
1_MQNVI 6_ NTVKG 11_ KALEV 16_ AEYLT 21_ PVLKE 26_ SKFKE 31_ TGVIT 36_ PEEFV 41_ AAGDH 46_ LVHHC 51_ PTWQW 56_ ATGEE 61_ LKVKA 66_ YLPTG 71_ KQFLV 76_ TKNVP 81_ CYKRC 86_ KQMEY 91_ SDELE 96_ AIIEE 101_ DDGDG 106_ GWVDT 111_ YHNTG 116_ ITGIT 121_ EAVKE 126_ ITLEN 131_ KDNIR 136_ LQDCS 141_ ALCEE 146_ EEDED 151_ EGEAA 156_ DMEEY 161_ EESGL 166_ LETDE 171_ ATLDT 176_ RKIVE 181_ ACKAK 186_ TDAGG 191_ EDAIL 196_ QTRTY 201_ DLYIT 206_ YDKYY 211_ QTPRL 216_ WLFGY 221_ DEQRQ 226_ PLTVE 231_ HMYED 236_ ISQDH 241_ VKKTV 246_ TIENH 251_ PHLPP 256_ PPMCS 261_ VHPCR 266_ HAEVM 271_ KKIIE 276_ TVAEG 281_ GGELG 286_ VHMYL 291_ LIFLK 296_ FVQAV 301_ IPTIE 306_YDYTR
1: E2 conjugating enzyme that catalyzes the covalent conjugation of the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to the amino group of phosphatidylethanolamine (PE)-containing lipids in the membrane resulting in membrane-bound ATG8-like proteins which is one of the key steps in the development of autophagic isolation membranes during autophagosome formation (PubMed:24191030, PubMed:33446636, PubMed:37252361). Cycles back and forth between binding to ATG7 for loading with the ATG8-like proteins and binding to E3 enzyme, composed of ATG12, ATG5 and ATG16L1 to promote ATG8-like proteins lipidation (PubMed:11825910, PubMed:12207896, PubMed:12890687, PubMed:16704426, PubMed:24186333). Also plays a role as a membrane curvature sensor that facilitates LC3/GABARAP lipidation by sensing local membrane stress associated with lipid-packing defects as occurs with high molar proportions of conical lipids or strident membrane curvature (By similarity). Interacts with negatively-charged membranes promoting membrane tethering and enhancing LC3/GABARAP lipidation (PubMed:29142222). Also acts as an autocatalytic E2-like enzyme by catalyzing the conjugation of ATG12 to itself in an ATG7-dependent manner, this complex thus formed, plays a role in mitochondrial homeostasis but not in autophagy (By similarity). ATG12-ATG3 conjugation promotes late endosome to lysosome trafficking and basal autophagosome maturation via its interaction with PDCD6IP (By similarity). ATG12-ATG3 conjugate is also formed upon viccina virus infection, leading to the disruption the cellular autophagy which is not necessary for vaccinia survival and proliferation (By similarity). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (By similarity)