NAD-dependent protein deacetylase sirtuin-3, mitochondrial (hSIRT3) (EC 2.3.1.286) (Regulatory protein SIR2 homolog 3) (SIR2-like protein 3)
1_MAFWG 6_ WRAAA 11_ ALRLW 16_ GRVVE 21_ RVEAG 26_ GGVGP 31_ FQACG 36_ CRLVL 41_ GGRDD 46_ VSAGL 51_ RGSHG 56_ ARGEP 61_ LDPAR 66_ PLQRP 71_ PRPEV 76_ PRAFR 81_ RQPRA 86_ AAPSF 91_ FFSSI 96_ KGGRR 101_ SISFS 106_ VGASS 111_ VVGSG 116_ GSSDK 121_ GKLSL 126_ QDVAE 131_ LIRAR 136_ ACQRV 141_ VVMVG 146_ AGIST 151_ PSGIP 156_ DFRSP 161_ GSGLY 166_ SNLQQ 171_ YDLPY 176_ PEAIF 181_ ELPFF 186_ FHNPK 191_ PFFTL 196_ AKELY 201_ PGNYK 206_ PNVTH 211_ YFLRL 216_ LHDKG 221_ LLLRL 226_ YTQNI 231_ DGLER 236_ VSGIP 241_ ASKLV 246_ EAHGT 251_ FASAT 256_ CTVCQ 261_ RPFPG 266_ EDIRA 271_ DVMAD 276_ RVPRC 281_ PVCTG 286_ VVKPD 291_ IVFFG 296_ EPLPQ 301_ RFLLH 306_ VVDFP 311_ MADLL 316_ LILGT 321_ SLEVE 326_ PFASL 331_ TEAVR 336_ SSVPR 341_ LLINR 346_ DLVGP 351_ LAWHP 356_ RSRDV 361_ AQLGD 366_ VVHGV 371_ ESLVE 376_ LLGWT 381_ EEMRD 386_ LVQRE 391_TGKLD
1: NAD-dependent protein deacetylase (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:19535340, PubMed:23283301, PubMed:24121500, PubMed:24252090). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:23283301, PubMed:24121500, PubMed:24252090, PubMed:38146092). Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA, MRPL12 and the ATP synthase subunit ATP5PO (PubMed:16788062, PubMed:18680753, PubMed:19535340, PubMed:24121500, PubMed:24252090, PubMed:38146092). Contributes to the regulation of the cellular energy metabolism (PubMed:24252090). Important for regulating tissue-specific ATP levels (PubMed:18794531). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed:23283301). Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity). Regulates hepatic lipogenesis (By similarity). Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance (By similarity). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by mediating delactylation of proteins, such as CCNE2 and 'Lys-16' of histone H4 (H4K16la) (PubMed:36896611, PubMed:37720100)