Ufm1-specific protease 2 (UfSP2) (EC 3.4.22.-)
1_MVISE 6_ SMDIL 11_ FRIRG 16_ GLDLA 21_ FQLAT 26_ PNEIF 31_ LKKAL 36_ KHVLS 41_ DLSTK 46_ LSSNA 51_ LVFRI 56_ CHSSV 61_ YIWPS 66_ SDINT 71_ IPGEL 76_ TDASA 81_ CKNIL 86_ RFIQF 91_ EPEED 96_ IKRKF 101_ MRKKD 106_ KKLSD 111_ MHQIV 116_ NIDLM 121_ LEMST 126_ SLAAV 131_ TPIIE 136_ RESGG 141_ HHYVN 146_ MTLPV 151_ DAVIS 156_ VAPEE 161_ TWGKV 166_ RKLLV 171_ DAIHN 176_ QLTDM 181_ EKCIL 186_ KYMKG 191_ TSIVV 196_ PEPLH 201_ FLLPG 206_ KKNLV 211_ TISYP 216_ SGIPD 221_ GQLQA 226_ YRKEL 231_ HDLFN 236_ LPHDR 241_ PYFKR 246_ SNAYH 251_ FPDEP 256_ YKDGY 261_ IRNPH 266_ TYLNP 271_ PNMET 276_ GMIYV 281_ VQGIY 286_ GYHHY 291_ MQDRI 296_ DDNGW 301_ GCAYR 306_ SLQTI 311_ CSWFK 316_ HQGYT 321_ ERSIP 326_ THREI 331_ QQALV 336_ DAGDK 341_ PATFV 346_ GSRQW 351_ IGSIE 356_ VQLVL 361_ NQLIG 366_ ITSKI 371_ LFVSQ 376_ GSEIA 381_ SQGRE 386_ LANHF 391_ QSEGT 396_ PVMIG 401_ GGVLA 406_ HTILG 411_ VAWNE 416_ ITGQI 421_ KFLIL 426_ DPHYT 431_ GAEDL 436_ QVILE 441_ KGWCG 446_ WKGPD 451_ FWNKD 456_ AYYNL 461_CLPQR
1: Thiol-dependent isopeptidase that specifically cleaves UFM1, a ubiquitin-like modifier protein, from conjugated proteins, such as CD274/PD-L1, CYB5R3, DDRGK1, MRE11, RPL26/uL24, TRIP4 and RPL26/uL24 (PubMed:25219498, PubMed:27351204, PubMed:27926783, PubMed:30783677, PubMed:31595041, PubMed:32160526, PubMed:33473208, PubMed:35394863, PubMed:35926457, PubMed:36543799, PubMed:36893266, PubMed:37795761, PubMed:38383785). While it is also able to mediate the processing of UFM1 precursors, a prerequisite for conjugation reactions, UFSP2 mainly acts as a protein deUFMylase that mediates deconjugation of UFM1 from target proteins (PubMed:27926783). Mediates deUFMylation of RPL26/uL24, a critical step to release the UFM1 ribosome E3 ligase (UREL) complex during the recycling of 60S ribosome subunits from the endoplasmic reticulum (PubMed:38383785). Catalyzes deUFMylation of TRIP4, regulating intracellular nuclear receptors transactivation and thereby regulate cell proliferation and differentiation (PubMed:25219498)