S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 (EC 4.1.3.44) (Radical S-adenosyl methionine and flavodoxin domain-containing protein 1) (tRNA wybutosine-synthesizing protein 1 homolog) (tRNA-yW-synthesizing protein)
1_MDPSA 6_ DTWDL 11_ FSPLI 16_ SLWIN 21_ RFYIY 26_ LGFAV 31_ SISLW 36_ ICVQI 41_ VIKTQ 46_ GKNLQ 51_ EKSVP 56_ KAAQD 61_ LMTNG 66_ YVSLQ 71_ EKDIF 76_ VSGVK 81_ IFYGS 86_ QTGTA 91_ KGFAT 96_ VLAEA 101_ VTSLD 106_ LPVAI 111_ INLKE 116_ YDPDD 121_ HLIEE 126_ VTSKN 131_ VCVFL 136_ VATYT 141_ DGLPT 146_ ESAEW 151_ FCKWL 156_ EEASI 161_ DFRFG 166_ KTYLK 171_ GMRYA 176_ VFGLG 181_ NSAYA 186_ SHFNK 191_ VGKNV 196_ DKWLW 201_ MLGAH 206_ RVMSR 211_ GEGDC 216_ DVVKS 221_ KHGSI 226_ EADFR 231_ AWKTK 236_ FISQL 241_ QALQK 246_ GERKK 251_ SCGGH 256_ CKKGK 261_ CESHQ 266_ HGSEE 271_ REEGS 276_ HEQDE 281_ LHHRD 286_ TEEEE 291_ PFESS 296_ SEEEF 301_ GGEDH 306_ QSLNS 311_ IVDVE 316_ DLGKI 321_ MDHVK 326_ KEKRE 331_ KEQQE 336_ EKSGL 341_ FRNMG 346_ RNEDG 351_ ERRAM 356_ ITPAL 361_ REALT 366_ KQGYQ 371_ LIGSH 376_ SGVKL 381_ CRWTK 386_ SMLRG 391_ RGGCY 396_ KHTFY 401_ GIESH 406_ RCMET 411_ TPSLA 416_ CANKC 421_ VFCWR 426_ HHTNP 431_ VGTEW 436_ RWKMD 441_ QPEMI 446_ LKEAI 451_ ENHQN 456_ MIKQF 461_ KGVPG 466_ VKAER 471_ FEEGM 476_ TVKHC 481_ ALSLV 486_ GEPIM 491_ YPEIN 496_ RFLKL 501_ LHQCK 506_ ISSFL 511_ VTNAQ 516_ FPAEI 521_ RNLEP 526_ VTQLY 531_ VSVDA 536_ STKDS 541_ LKKID 546_ RPLFK 551_ DFWQR 556_ FLDSL 561_ KALAV 566_ KQQRT 571_ VYRLT 576_ LVKAW 581_ NVDEL 586_ QAYAQ 591_ LVSLG 596_ NPDFI 601_ EVKGV 606_ TYCGE 611_ SSASS 616_ LTMAH 621_ VPWHE 626_ EVVQF 631_ VHELV 636_ DLIPE 641_ YEIAC 646_ EHEHS 651_ NCLLI 656_ AHRKF 661_ KIGGE 666_ WWTWI 671_ DYNRF 676_ QELIQ 681_ EYEDS 686_ GGSKT 691_ FSAKD 696_ YMART 701_ PHWAL 706_ FGASE 711_ RGFDP 716_ KDTRH 721_ QRKNK 726_SKAIS
1: Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis (By similarity)