DNA-directed RNA polymerase III subunit RPC2 (RNA polymerase III subunit C2) (EC 2.7.7.6) (C128) (DNA-directed RNA polymerase III 127.6 kDa polypeptide) (DNA-directed RNA polymerase III subunit B)
1_MDVLA 6_ EEFGN 11_ LTPEQ 16_ LAAPI 21_ PTVEE 26_ KWRLL 31_ PAFLK 36_ VKGLV 41_ KQHID 46_ SFNYF 51_ INVEI 56_ KKIMK 61_ ANEKV 66_ TSDAD 71_ PMWYL 76_ KYLNI 81_ YVGLP 86_ DVEES 91_ FNVTR 96_ PVSPH 101_ ECRLR 106_ DMTYS 111_ APITV 116_ DIEYT 121_ RGSQR 126_ IIRNA 131_ LPIGR 136_ MPIML 141_ RSSNC 146_ VLTGK 151_ TPAEF 156_ AKLNE 161_ CPLDP 166_ GGYFI 171_ VKGVE 176_ KVILI 181_ QEQLS 186_ KNRII 191_ VEADR 196_ KGAVG 201_ ASVTS 206_ STHEK 211_ KSRTN 216_ MAVKQ 221_ GRFYL 226_ RHNTL 231_ SEDIP 236_ IVIIF 241_ KAMGV 246_ ESDQE 251_ IVQMI 256_ GTEEH 261_ VMAAF 266_ GPSLE 271_ ECQKA 276_ QIFTQ 281_ MQALK 286_ YIGNK 291_ VRRQR 296_ MWGGG 301_ PKKTK 306_ IEEAR 311_ ELLAS 316_ TILTH 321_ VPVKE 326_ FNFRA 331_ KCIYT 336_ AVMVR 341_ RVILA 346_ QGDNK 351_ VDDRD 356_ YYGNK 361_ RLELA 366_ GQLLS 371_ LLFED 376_ LFKKF 381_ NSEMK 386_ KIADQ 391_ VIPKQ 396_ RAAQF 401_ DVVKH 406_ MRQDQ 411_ ITNGM 416_ VNAIS 421_ TGNWS 426_ LKRFK 431_ MDRQG 436_ VTQVL 441_ SRLSY 446_ ISALG 451_ MMTRI 456_ SSQFE 461_ KTRKV 466_ SGPRS 471_ LQPSQ 476_ WGMLC 481_ PSDTP 486_ EGEAC 491_ GLVKN 496_ LALMT 501_ HITTD 506_ MEDGP 511_ IVKLA 516_ SNLGV 521_ EDVNL 526_ LCGEE 531_ LSYPN 536_ VFLVF 541_ LNGNI 546_ LGVIR 551_ DHKKL 556_ VNTFR 561_ LMRRA 566_ GYINE 571_ FVSIS 576_ TNLTD 581_ RCVYI 586_ SSDGG 591_ RLCRP 596_ YIIVK 601_ KQKPA 606_ VTNKH 611_ MEELA 616_ QGYRN 621_ FEDFL 626_ HESLV 631_ EYLDV 636_ NEEND 641_ CNIAL 646_ YEHTI 651_ NKDTT 656_ HLEIE 661_ PFTLL 666_ GVCAG 671_ LIPYP 676_ HHNQS 681_ PRNTY 686_ QCAMG 691_ KQAMG 696_ TIGYN 701_ QRNRI 706_ DTLMY 711_ LLAYP 716_ QKPMV 721_ KTKTI 726_ ELIEF 731_ EKLPA 736_ GQNAT 741_ VAVMS 746_ YSGYD 751_ IEDAL 756_ VLNKA 761_ SLDRG 766_ FGRCL 771_ VYKNA 776_ KCTLK 781_ RYTNQ 786_ TFDKV 791_ MGPML 796_ DAATR 801_ KPIWR 806_ HEILD 811_ ADGIC 816_ SPGEK 821_ VENKQ 826_ VLVNK 831_ SMPTV 836_ TQIPL 841_ EGSNV 846_ PQQPQ 851_ YKDVP 856_ ITYKG 861_ ATDSY 866_ IEKVM 871_ ISSNA 876_ EDAFL 881_ IKMLL 886_ RQTRR 891_ PEIGD 896_ KFSSR 901_ HGQKG 906_ VCGLI 911_ VPQED 916_ MPFCD 921_ SGICP 926_ DIIMN 931_ PHGFP 936_ SRMTV 941_ GKLIE 946_ LLAGK 951_ AGVLD 956_ GRFHY 961_ GTAFG 966_ GSKVK 971_ DVCED 976_ LVRHG 981_ YNYLG 986_ KDYVT 991_ SGITG 996_ EPLEA 1001_ YIYFG 1006_ PVYYQ 1011_ KLKHM 1016_ VLDKM 1021_ HARAR 1026_ GPRAV 1031_ LTRQP 1036_ TEGRS 1041_ RDGGL 1046_ RLGEM 1051_ ERDCL 1056_ IGYGA 1061_ SMLLL 1066_ ERLMI 1071_ SSDAF 1076_ EVDVC 1081_ GQCGL 1086_ LGYSG 1091_ WCHYC 1096_ KSSCH 1101_ VSSLR 1106_ IPYAC 1111_ KLLFQ 1116_ ELQSM 1121_ NIIPR 1126_LKLSK
1: Catalytic core component of RNA polymerase III (Pol III), a DNA-dependent RNA polymerase which synthesizes small non-coding RNAs using the four ribonucleoside triphosphates as substrates. Synthesizes 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci (PubMed:20413673, PubMed:33558766). Pol III-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol III is recruited to DNA promoters type I, II or III with the help of general transcription factors and other specific initiation factors. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Transcription termination involves the release of the RNA transcript and polymerase from the DNA (PubMed:20413673, PubMed:33335104, PubMed:33558764, PubMed:33558766, PubMed:33674783, PubMed:34675218). Forms Pol III active center together with the largest subunit POLR3A/RPC1. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. Appends one nucleotide at a time to the 3' end of the nascent RNA, with POLR3A/RPC1 contributing a Mg(2+)-coordinating DxDGD motif, and POLR3B/RPC2 participating in the coordination of a second Mg(2+) ion and providing lysine residues believed to facilitate Watson-Crick base pairing between the incoming nucleotide and template base. Typically, Mg(2+) ions direct a 5' nucleoside triphosphate to form a phosphodiester bond with the 3' hydroxyl of the preceding nucleotide of the nascent RNA, with the elimination of pyrophosphate (PubMed:19609254, PubMed:20413673, PubMed:33335104, PubMed:33558764, PubMed:33674783, PubMed:34675218). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway