Hypoxia-inducible factor 1-alpha inhibitor (EC 1.14.11.30) (EC 1.14.11.n4) (Factor inhibiting HIF-1) (FIH-1) (Hypoxia-inducible factor asparagine hydroxylase)
1_MAATA 6_ AEAVA 11_ SGSGE 16_ PREEA 21_ GALGP 26_ AWDES 31_ QLRSY 36_ SFPTR 41_ PIPRL 46_ SQSDP 51_ RAEEL 56_ IENEE 61_ PVVLT 66_ DTNLV 71_ YPALK 76_ WDLEY 81_ LQENI 86_ GNGDF 91_ SVYSA 96_ STHKF 101_ LYYDE 106_ KKMAN 111_ FQNFK 116_ PRSNR 121_ EEMKF 126_ HEFVE 131_ KLQDI 136_ QQRGG 141_ EERLY 146_ LQQTL 151_ NDTVG 156_ RKIVM 161_ DFLGF 166_ NWNWI 171_ NKQQG 176_ KRGWG 181_ QLTSN 186_ LLLIG 191_ MEGNV 196_ TPAHY 201_ DEQQN 206_ FFAQI 211_ KGYKR 216_ CILFP 221_ PDQFE 226_ CLYPY 231_ PVHHP 236_ CDRQS 241_ QVDFD 246_ NPDYE 251_ RFPNF 256_ QNVVG 261_ YETVV 266_ GPGDV 271_ LYIPM 276_ YWWHH 281_ IESLL 286_ NGGIT 291_ ITVNF 296_ WYKGA 301_ PTPKR 306_ IEYPL 311_ KAHQK 316_ VAIMR 321_ NIEKM 326_ LGEAL 331_ GNPQE 336_ VGPLL 341_NTMIK
1: Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation