Gem-associated protein 8 (Gemin-8) (Protein FAM51A1)
1_MAAVK 6_ ASTSK 11_ ATRPW 16_ YSHPV 21_ YARYW 26_ QHYHQ 31_ AMAWM 36_ QSHHN 41_ AYRKA 46_ VESCF 51_ NLPWY 56_ LPSAL 61_ LPQSS 66_ YDNEA 71_ AYPQS 76_ FYDHH 81_ VAWQD 86_ YPCSS 91_ SHFRR 96_ SGQHP 101_ RYSSR 106_ IQAST 111_ KEDQA 116_ LSKEE 121_ EMETE 126_ SDAEV 131_ ECDLS 136_ NMEIT 141_ EELRQ 146_ YFAET 151_ ERHRE 156_ ERRRQ 161_ QQLDA 166_ ERLDS 171_ YVNAD 176_ HDLYC 181_ NTRRS 186_ VEAPT 191_ ERPGE 196_ RRQAE 201_ MKRLY 206_ GDSAA 211_ KIQAM 216_ EAAVQ 221_ LSFDK 226_ HCDRK 231_ QPKYW 236_PVIPL
1: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP