24-hydroxycholesterol 7-alpha-hydroxylase (EC 1.14.14.26) (Cytochrome P450 39A1) (hCYP39A1) (Oxysterol 7-alpha-hydroxylase)
1_MELIS 6_ PTVII 11_ ILGCL 16_ ALFLL 21_ LQRKN 26_ LRRPP 31_ CIKGW 36_ IPWIG 41_ VGFEF 46_ GKAPL 51_ EFIEK 56_ ARIKY 61_ GPIFT 66_ VFAMG 71_ NRMTF 76_ VTEEE 81_ GINVF 86_ LKSKK 91_ VDFEL 96_ AVQNI 101_ VYRTA 106_ SIPKN 111_ VFLAL 116_ HEKLY 121_ IMLKG 126_ KMGTV 131_ NLHQF 136_ TGQLT 141_ EELHE 146_ QLENL 151_ GTHGT 156_ MDLNN 161_ LVRHL 166_ LYPVT 171_ VNMLF 176_ NKSLF 181_ STNKK 186_ KIKEF 191_ HQYFQ 196_ VYDED 201_ FEYGS 206_ QLPEC 211_ LLRNW 216_ SKSKK 221_ WFLEL 226_ FEKNI 231_ PDIKA 236_ CKSAK 241_ DNSMT 246_ LLQAT 251_ LDIVE 256_ TETSK 261_ ENSPN 266_ YGLLL 271_ LWASL 276_ SNAVP 281_ VAFWT 286_ LAYVL 291_ SHPDI 296_ HKAIM 301_ EGISS 306_ VFGKA 311_ GKDKI 316_ KVSED 321_ DLENL 326_ LLIKW 331_ CVLET 336_ IRLKA 341_ PGVIT 346_ RKVVK 351_ PVEIL 356_ NYIIP 361_ SGDLL 366_ MLSPF 371_ WLHRN 376_ PKYFP 381_ EPELF 386_ KPERW 391_ KKANL 396_ EKHSF 401_ LDCFM 406_ AFGSG 411_ KFQCP 416_ ARWFA 421_ LLEVQ 426_ MCIIL 431_ ILYKY 436_ DCSLL 441_ DPLPK 446_ QSYLH 451_ LVGVP 456_ QPEGQ 461_CRIEY
1: A cytochrome P450 monooxygenase involved in neural cholesterol clearance through bile acid synthesis (PubMed:10748047, PubMed:25201972). Catalyzes 7-alpha hydroxylation of (24S)-hydroxycholesterol, a neural oxysterol that is metabolized to bile acids in the liver (PubMed:10748047, PubMed:25201972). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:10748047, PubMed:25201972)