Spastin (EC 5.6.1.1) (Spastic paraplegia 4 protein)
1_MNSPG 6_ GRGKK 11_ KGSGG 16_ ASNPV 21_ PPRPP 26_ PPCLA 31_ PAPPA 36_ AGPAP 41_ PPESP 46_ HKRNL 51_ YYFSY 56_ PLFVG 61_ FALLR 66_ LVAFH 71_ LGLLF 76_ VWLCQ 81_ RFSRA 86_ LMAAK 91_ RSSGA 96_ APAPA 101_ SASAP 106_ APVPG 111_ GEAER 116_ VRVFH 121_ KQAFE 126_ YISIA 131_ LRIDE 136_ DEKAG 141_ QKEQA 146_ VEWYK 151_ KGIEE 156_ LEKGI 161_ AVIVT 166_ GQGEQ 171_ CERAR 176_ RLQAK 181_ MMTNL 186_ VMAKD 191_ RLQLL 196_ EKMQP 201_ VLPFS 206_ KSQTD 211_ VYNDS 216_ TNLAC 221_ RNGHL 226_ QSESG 231_ AVPKR 236_ KDPLT 241_ HTSNS 246_ LPRSK 251_ TVMKT 256_ GSAGL 261_ SGHHR 266_ APSYS 271_ GLSMV 276_ SGVKQ 281_ GSGPA 286_ PTTHK 291_ GTPKT 296_ NRTNK 301_ PSTPT 306_ TATRK 311_ KKDLK 316_ NFRNV 321_ DSNLA 326_ NLIMN 331_ EIVDN 336_ GTAVK 341_ FDDIA 346_ GQDLA 351_ KQALQ 356_ EIVIL 361_ PSLRP 366_ ELFTG 371_ LRAPA 376_ RGLLL 381_ FGPPG 386_ NGKTM 391_ LAKAV 396_ AAESN 401_ ATFFN 406_ ISAAS 411_ LTSKY 416_ VGEGE 421_ KLVRA 426_ LFAVA 431_ RELQP 436_ SIIFI 441_ DEVDS 446_ LLCER 451_ REGEH 456_ DASRR 461_ LKTEF 466_ LIEFD 471_ GVQSA 476_ GDDRV 481_ LVMGA 486_ TNRPQ 491_ ELDEA 496_ VLRRF 501_ IKRVY 506_ VSLPN 511_ EETRL 516_ LLLKN 521_ LLCKQ 526_ GSPLT 531_ QKELA 536_ QLARM 541_ TDGYS 546_ GSDLT 551_ ALAKD 556_ AALGP 561_ IRELK 566_ PEQVK 571_ NMSAS 576_ EMRNI 581_ RLSDF 586_ TESLK 591_ KIKRS 596_ VSPQT 601_ LEAYI 606_ RWNKD 611_FGDTT
1: ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:11809724, PubMed:15716377, PubMed:16219033, PubMed:17389232, PubMed:20530212, PubMed:22637577, PubMed:26875866). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Severing activity is not dependent on tubulin acetylation or detyrosination (PubMed:26875866). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex (PubMed:19000169, PubMed:21310966, PubMed:26040712). Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex (PubMed:21310966). Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712). Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling (PubMed:23897888). Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (PubMed:23897888). Probably plays a role in axon growth and the formation of axonal branches (PubMed:15716377)
2: Involved in lipid metabolism by regulating the size and distribution of lipid droplets