tRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.33) (Methyltransferase-like protein 1) (mRNA (guanine-N(7)-)-methyltransferase) (EC 2.1.1.-) (miRNA (guanine-N(7)-)-methyltransferase) (EC 2.1.1.-) (tRNA (guanine(46)-N(7))-methyltransferase) (tRNA(m7G46)-methyltransferase)
1_MAAET 6_ RNVAG 11_ AEAPP 16_ PQKRY 21_ YRQRA 26_ HSNPM 31_ ADHTL 36_ RYPVK 41_ PEEMD 46_ WSELY 51_ PEFFA 56_ PLTQN 61_ QSHDD 66_ PKDKK 71_ EKRAQ 76_ AQVEF 81_ ADIGC 86_ GYGGL 91_ LVELS 96_ PLFPD 101_ TLILG 106_ LEIRV 111_ KVSDY 116_ VQDRI 121_ RALRA 126_ APAGG 131_ FQNIA 136_ CLRSN 141_ AMKHL 146_ PNFFY 151_ KGQLT 156_ KMFFL 161_ FPDPH 166_ FKRTK 171_ HKWRI 176_ ISPTL 181_ LAEYA 186_ YVLRV 191_ GGLVY 196_ TITDV 201_ LELHD 206_ WMCTH 211_ FEEHP 216_ LFERV 221_ PLEDL 226_ SEDPV 231_ VGHLG 236_ TSTEE 241_ GKKVL 246_ RNGGK 251_ NFPAI 256_ FRRIQ 261_ DPVLQ 266_ AVTSQ 271_TSLPG
1: Catalytic component of METTL1-WDR4 methyltransferase complex that mediates the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:12403464, PubMed:31031083, PubMed:31031084, PubMed:36599982, PubMed:36599985, PubMed:37369656, PubMed:37379838). Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop (PubMed:12403464, PubMed:34352206, PubMed:34352207, PubMed:36599982, PubMed:36599985, PubMed:37369656). M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay (PubMed:36599982, PubMed:36599985). Also acts as a methyltransferase for a subset of internal N(7)-methylguanine in mRNAs (PubMed:31031084, PubMed:37379838). Internal N(7)-methylguanine methylation of mRNAs in response to stress promotes their relocalization to stress granules, thereby suppressing their translation (PubMed:31031084, PubMed:37379838). Also methylates a specific subset of miRNAs, such as let-7 (PubMed:31031083). N(7)-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA) (PubMed:31031083). Acts as a regulator of embryonic stem cell self-renewal and differentiation (By similarity)