Peflin (PEF protein with a long N-terminal hydrophobic domain) (Penta-EF hand domain-containing protein 1)
1_MASYP 6_ YRQGC 11_ PGAAG 16_ QAPGA 21_ PPGSY 26_ YPGPP 31_ NSGGQ 36_ YGSGL 41_ PPGGG 46_ YGGPA 51_ PGGPY 56_ GPPAG 61_ GGPYG 66_ HPNPG 71_ MFPSG 76_ TPGGP 81_ YGGAA 86_ PGGPY 91_ GQPPP 96_ SSYGA 101_ QQPGL 106_ YGQGG 111_ APPNV 116_ DPEAY 121_ SWFQS 126_ VDSDH 131_ SGYIS 136_ MKELK 141_ QALVN 146_ CNWSS 151_ FNDET 156_ CLMMI 161_ NMFDK 166_ TKSGR 171_ IDVYG 176_ FSALW 181_ KFIQQ 186_ WKNLF 191_ QQYDR 196_ DRSGS 201_ ISYTE 206_ LQQAL 211_ SQMGY 216_ NLSPQ 221_ FTQLL 226_ VSRYC 231_ PRSAN 236_ PAMQL 241_ DRFIQ 246_ VCTQL 251_ QVLTE 256_ AFREK 261_ DTAVQ 266_ GNIRL 271_ SFEDF 276_VTMTA
1: Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats (PubMed:27716508). In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification (PubMed:27716508). Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium (PubMed:27716508). Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6 (PubMed:11278427). Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity)