Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase (EC 2.1.1.205) (2'-O-ribose RNA methyltransferase TRM7 homolog) (Protein ftsJ homolog 1)
1_MGRTS 6_ KDKRD 11_ VYYRL 16_ AKENG 21_ WRARS 26_ AFKLL 31_ QLDKE 36_ FQLFQ 41_ GVTRA 46_ VDLCA 51_ APGSW 56_ SQVLS 61_ QKIGG 66_ QGSGH 71_ VVAVD 76_ LQAMA 81_ PLPGV 86_ VQIQG 91_ DITQL 96_ STAKE 101_ IIQHF 106_ KGCPA 111_ DLVVC 116_ DGAPD 121_ VTGLH 126_ DVDEY 131_ MQAQL 136_ LLAAL 141_ NIATH 146_ VLKPG 151_ GCFVA 156_ KIFRG 161_ RDVTL 166_ LYSQL 171_ QVFFS 176_ SVLCA 181_ KPRSS 186_ RNSSI 191_ EAFAV 196_ CQGYD 201_ PPEGF 206_ IPDLS 211_ KPLLD 216_ HSYDP 221_ DFNQL 226_ DGPTR 231_ IIVPF 236_ VTCGD 241_ LSSYD 246_ SDRSY 251_ PLDLE 256_ GGSEY 261_ KYTPP 266_ TQPPI 271_ SPPYQ 276_ EACTL 281_ KRKGQ 286_ LAKEI 291_ RPQDC 296_ PISRV 301_ DTFPQ 306_ PLAAP 311_ QCHTL 316_ LAPEM 321_EDNEM
1: Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs (PubMed:25404562, PubMed:26310293, PubMed:32198346, PubMed:32558197, PubMed:33771871, PubMed:36720500). Requisite for faithful cytoplasmic translation (PubMed:32393790). Requires THADA for methylation of the nucleotide at position 32 of the anticodon loop of substrate tRNAs (PubMed:25404562, PubMed:26310293). Requires WDR6 for methylation of the nucleotide at position 34 of the anticodon loop of substrate tRNAs (PubMed:32558197, PubMed:33771871). Promotes translation efficiency of the UUU codon (PubMed:32558197). Plays a role in neurogenesis (PubMed:36720500). Required for expression of genes involved in neurogenesis, mitochondrial translation and energy generation, and lipid biosynthesis (PubMed:33771871, PubMed:36720500). Requisite for RNA-mediated gene silencing (PubMed:36720500). May modify position 32 in tRNA(Arg(ACG)), tRNA(Arg(CCG)), tRNA(Arg(UCG)), tRNA(Cys(GCA)), tRNA(Cys(ACA)), tRNA(Gln(CUG)), tRNA(Gln(UUG)), tRNA(Gly(CCC)), tRNA(Leu(CAG))/tRNA(Leu(CAA)), tRNA(Leu(A/IAG)), tRNA(Leu(UAG)), tRNA(Phe(GAA)), tRNA(Pro(AGG))/tRNA(Pro(CGG))/tRNA(Pro(UGG)) and tRNA(Trp(CCA)), and position 34 in tRNA(Phe(GAA)), tRNA(Leu(CAA)), tRNA(Sec(UCA)), and tRNA(Trp(CCA)) (PubMed:26310293, PubMed:32198346, PubMed:32558197, PubMed:33771871, PubMed:36720500)