STE20/SPS1-related proline-alanine-rich protein kinase (Ste-20-related kinase) (EC 2.7.11.1) (DCHT) (Serine/threonine-protein kinase 39)
1_MAEPS 6_ GSPVH 11_ VQLPQ 16_ QAAPV 21_ TAAAA 26_ AAPAA 31_ ATAAP 36_ APAAP 41_ AAPAP 46_ APAPA 51_ AQAVG 56_ WPICR 61_ DAYEL 66_ QEVIG 71_ SGATA 76_ VVQAA 81_ LCKPR 86_ QERVA 91_ IKRIN 96_ LEKCQ 101_ TSMDE 106_ LLKEI 111_ QAMSQ 116_ CSHPN 121_ VVTYY 126_ TSFVV 131_ KDELW 136_ LVMKL 141_ LSGGS 146_ MLDII 151_ KYIVN 156_ RGEHK 161_ NGVLE 166_ EAIIA 171_ TILKE 176_ VLEGL 181_ DYLHR 186_ NGQIH 191_ RDLKA 196_ GNILL 201_ GEDGS 206_ VQIAD 211_ FGVSA 216_ FLATG 221_ GDVTR 226_ NKVRK 231_ TFVGT 236_ PCWMA 241_ PEVME 246_ QVRGY 251_ DFKAD 256_ MWSFG 261_ ITAIE 266_ LATGA 271_ APYHK 276_ YPPMK 281_ VLMLT 286_ LQNDP 291_ PTLET 296_ GVEDK 301_ EMMKK 306_ YGKSF 311_ RKLLS 316_ LCLQK 321_ DPSKR 326_ PTAAE 331_ LLKCK 336_ FFQKA 341_ KNREY 346_ LIEKL 351_ LTRTP 356_ DIAQR 361_ AKKVR 366_ RVPGS 371_ SGHLH 376_ KTEDG 381_ DWEWS 386_ DDEMD 391_ EKSEE 396_ GKAAF 401_ SQEKS 406_ RRVKE 411_ ENPEI 416_ AVSAS 421_ TIPEQ 426_ IQSLS 431_ VHDSQ 436_ GPPNA 441_ NEDYR 446_ EASSC 451_ AVNLV 456_ LRLRN 461_ SRKEL 466_ NDIRF 471_ EFTPG 476_ RDTAD 481_ GVSQE 486_ LFSAG 491_ LVDGH 496_ DVVIV 501_ AANLQ 506_ KIVDD 511_ PKALK 516_ TLTFK 521_ LASGC 526_ DGSEI 531_ PDEVK 536_LIGFA
1: Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure (PubMed:16669787, PubMed:18270262, PubMed:21321328, PubMed:34289367). Specifically recognizes and binds proteins with a RFXV motif (PubMed:16669787, PubMed:21321328). Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4): following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed:21321328). Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases (PubMed:12740379, PubMed:16669787, PubMed:21321328). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux (PubMed:16669787, PubMed:19665974, PubMed:21321328). Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4 (PubMed:18270262). Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities (By similarity). Phosphorylates RELT (By similarity)