NADPH-dependent diflavin oxidoreductase 1 (EC 1.18.1.-) (NADPH-dependent FMN and FAD-containing oxidoreductase) (Novel reductase 1)
1_MPSPQ 6_ LLVLF 11_ GSQTG 16_ TAQDV 21_ SERLG 26_ REARR 31_ RRLGC 36_ RVQAL 41_ DSYPV 46_ VNLIN 51_ EPLVI 56_ FVCAT 61_ TGQGD 66_ PPDNM 71_ KNFWR 76_ FIFRK 81_ NLPST 86_ ALCQM 91_ DFAVL 96_ GLGDS 101_ SYAKF 106_ NFVAK 111_ KLHRR 116_ LLQLG 121_ GSALL 126_ PVCLG 131_ DDQHE 136_ LGPDA 141_ AVDPW 146_ LRDLW 151_ DRVLG 156_ LYPPP 161_ PGLTE 166_ IPPGV 171_ PLPSK 176_ FTLLF 181_ LQEAP 186_ STGSE 191_ GQRVA 196_ HPGSQ 201_ EPPSE 206_ SKPFL 211_ APMIS 216_ NQRVT 221_ GPSHF 226_ QDVRL 231_ IEFDI 236_ LGSGI 241_ SFAAG 246_ DVVLI 251_ QPSNS 256_ AAHVQ 261_ RFCQV 266_ LGLDP 271_ DQLFM 276_ LQPRE 281_ PDVSS 286_ PTRLP 291_ QPCSM 296_ RHLVS 301_ HYLDI 306_ ASVPR 311_ RSFFE 316_ LLACL 321_ SLHEL 326_ EREKL 331_ LEFSS 336_ AQGQE 341_ ELFEY 346_ CNRPR 351_ RTILE 356_ VLCDF 361_ PHTAA 366_ AIPPD 371_ YLLDL 376_ IPVIR 381_ PRAFS 386_ IASSL 391_ LTHPS 396_ RLQIL 401_ VAVVQ 406_ FQTRL 411_ KEPRR 416_ GLCSS 421_ WLASL 426_ DPGQG 431_ PVRVP 436_ LWVRP 441_ GSLAF 446_ PETPD 451_ TPVIM 456_ VGPGT 461_ GVAPF 466_ RAAIQ 471_ ERVAQ 476_ GQTGN 481_ FLFFG 486_ CRWRD 491_ QDFYW 496_ EAEWQ 501_ ELEKR 506_ DCLTL 511_ IPAFS 516_ REQEQ 521_ KVYVQ 526_ HRLRE 531_ LGSLV 536_ WELLD 541_ RQGAY 546_ FYLAG 551_ NAKSM 556_ PADVS 561_ EALMS 566_ IFQEE 571_ GGLCS 576_ PDAAA 581_ YLARL 586_ QQTRR 591_FQTET
1: NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery (PubMed:10625700, PubMed:15900210, PubMed:20802492, PubMed:23596212, PubMed:28648056). Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of CIAPIN1, another key component of the CIA machinery (PubMed:20802492, PubMed:23596212, PubMed:28648056). In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins (PubMed:20802492, PubMed:23596212). It can also reduce the [2Fe-2S] cluster of CISD1 and activate this protein implicated in Fe/S cluster repair (PubMed:28648056). In vitro can fully activate methionine synthase/MTR in the presence of soluble cytochrome b5/CYB5A (PubMed:12871938)