Cleavage and polyadenylation specificity factor subunit 3 (EC 3.1.27.-) (Cleavage and polyadenylation specificity factor 73 kDa subunit) (CPSF 73 kDa subunit) (mRNA 3'-end-processing endonuclease CPSF-73)
1_MSAIP 6_ AEESD 11_ QLLIR 16_ PLGAG 21_ QEVGR 26_ SCIIL 31_ EFKGR 36_ KIMLD 41_ CGIHP 46_ GLEGM 51_ DALPY 56_ IDLID 61_ PAEID 66_ LLLIS 71_ HFHLD 76_ HCGAL 81_ PWFLQ 86_ KTSFK 91_ GRTFM 96_ THATK 101_ AIYRW 106_ LLSDY 111_ VKVSN 116_ ISADD 121_ MLYTE 126_ TDLEE 131_ SMDKI 136_ ETINF 141_ HEVKE 146_ VAGIK 151_ FWCYH 156_ AGHVL 161_ GAAMF 166_ MIEIA 171_ GVKLL 176_ YTGDF 181_ SRQED 186_ RHLMA 191_ AEIPN 196_ IKPDI 201_ LIIES 206_ TYGTH 211_ IHEKR 216_ EEREA 221_ RFCNT 226_ VHDIV 231_ NRGGR 236_ GLIPV 241_ FALGR 246_ AQELL 251_ LILDE 256_ YWQNH 261_ PELHD 266_ IPIYY 271_ ASSLA 276_ KKCMA 281_ VYQTY 286_ VNAMN 291_ DKIRK 296_ QININ 301_ NPFVF 306_ KHISN 311_ LKSMD 316_ HFDDI 321_ GPSVV 326_ MASPG 331_ MMQSG 336_ LSREL 341_ FESWC 346_ TDKRN 351_ GVIIA 356_ GYCVE 361_ GTLAK 366_ HIMSE 371_ PEEIT 376_ TMSGQ 381_ KLPLK 386_ MSVDY 391_ ISFSA 396_ HTDYQ 401_ QTSEF 406_ IRALK 411_ PPHVI 416_ LVHGE 421_ QNEMA 426_ RLKAA 431_ LIREY 436_ EDNDE 441_ VHIEV 446_ HNPRN 451_ TEAVT 456_ LNFRG 461_ EKLAK 466_ VMGFL 471_ ADKKP 476_ EQGQR 481_ VSGIL 486_ VKRNF 491_ NYHIL 496_ SPCDL 501_ SNYTD 506_ LAMST 511_ VKQTQ 516_ AIPYT 521_ GPFNL 526_ LCYQL 531_ QKLTG 536_ DVEEL 541_ EIQEK 546_ PALKV 551_ FKNIT 556_ VIQEP 561_ GMVVL 566_ EWLAN 571_ PSNDM 576_ YADTV 581_ TTVIL 586_ EVQSN 591_ PKIRK 596_ GAVQK 601_ VSKKL 606_ EMHVY 611_ SKRLE 616_ IMLQD 621_ IFGED 626_ CVSVK 631_ DDSIL 636_ SVTVD 641_ GKTAN 646_ LNLET 651_ RTVEC 656_ EEGSE 661_ DDESL 666_ REMVE 671_ LAAQR 676_LYEAL
1: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as an mRNA 3'-end-processing endonuclease (PubMed:30507380). Also involved in the histone 3'-end pre-mRNA processing (PubMed:30507380). U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for entering/progressing through S-phase of the cell cycle (PubMed:30507380). Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1 (By similarity). Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a (By similarity). Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively (By similarity)