F-box only protein 5 (Early mitotic inhibitor 1)
1_MSRRP 6_ CSCAL 11_ RPPRC 16_ SCSAS 21_ PSAVT 26_ AAGRP 31_ RPSDS 36_ CKEES 41_ STLSV 46_ KMKCD 51_ FNCNH 56_ VHSGL 61_ KLVKP 66_ DDIGR 71_ LVSYT 76_ PAYLE 81_ GSCKD 86_ CIKDY 91_ ERLSC 96_ IGSPI 101_ VSPRI 106_ VQLET 111_ ESKRL 116_ HNKEN 121_ QHVQQ 126_ TLNST 131_ NEIEA 136_ LETSR 141_ LYEDS 146_ GYSSF 151_ SLQSG 156_ LSEHE 161_ EGSLL 166_ EENFG 171_ DSLQS 176_ CLLQI 181_ QSPDQ 186_ YPNKN 191_ LLPVL 196_ HFEKV 201_ VCSTL 206_ KKNAK 211_ RNPKV 216_ DREML 221_ KEIIA 226_ RGNFR 231_ LQNII 236_ GRKMG 241_ LECVD 246_ ILSEL 251_ FRRGL 256_ RHVLA 261_ TILAQ 266_ LSDMD 271_ LINVS 276_ KVSTT 281_ WKKIL 286_ EDDKG 291_ AFQLY 296_ SKAIQ 301_ RVTEN 306_ NNKFS 311_ PHAST 316_ REYVM 321_ FRTPL 326_ ASVQK 331_ SAAQT 336_ SLKKD 341_ AQTKL 346_ SNQGD 351_ QKGST 356_ YSRHN 361_ EFSEV 366_ AKTLK 371_ KNESL 376_ KACIR 381_ CNSPA 386_ KYDCY 391_ LQRAT 396_ CKREG 401_ CGFDY 406_ CTKCL 411_ CNYHT 416_ TKDCS 421_ DGKLL 426_ KASCK 431_ IGPLP 436_ GTKKS 441_KKNLR
1: Regulator of APC activity during mitotic and meiotic cell cycle (PubMed:16921029, PubMed:17234884, PubMed:17485488, PubMed:17875940, PubMed:23708001, PubMed:23708605). During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex (PubMed:16921029, PubMed:17234884, PubMed:17485488, PubMed:17875940, PubMed:23708001, PubMed:23708605, PubMed:29875408). During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase (PubMed:29875408). Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment (PubMed:29875408). As APC inhibitor, prevents the degradation of APC substrates at multiple levels: by interacting with APC and blocking access of APC substrates to the D-box coreceptor, formed by FZR1 and ANAPC10; by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities (PubMed:16921029, PubMed:23708001, PubMed:23708605). Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence (PubMed:17234884, PubMed:17485488, PubMed:17875940). During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (By similarity). Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (By similarity). Promotes migration and osteogenic differentiation of mesenchymal stem cells (PubMed:29850565)