Protein argonaute-2 (Argonaute2) (hAgo2) (EC 3.1.26.n2) (Argonaute RISC catalytic component 2) (Eukaryotic translation initiation factor 2C 2) (eIF-2C 2) (eIF2C 2) (PAZ Piwi domain protein) (PPD) (Protein slicer)
1_MYSGA 6_ GPALA 11_ PPAPP 16_ PPIQG 21_ YAFKP 26_ PPRPD 31_ FGTSG 36_ RTIKL 41_ QANFF 46_ EMDIP 51_ KIDIY 56_ HYELD 61_ IKPEK 66_ CPRRV 71_ NREIV 76_ EHMVQ 81_ HFKTQ 86_ IFGDR 91_ KPVFD 96_ GRKNL 101_ YTAMP 106_ LPIGR 111_ DKVEL 116_ EVTLP 121_ GEGKD 126_ RIFKV 131_ SIKWV 136_ SCVSL 141_ QALHD 146_ ALSGR 151_ LPSVP 156_ FETIQ 161_ ALDVV 166_ MRHLP 171_ SMRYT 176_ PVGRS 181_ FFTAS 186_ EGCSN 191_ PLGGG 196_ REVWF 201_ GFHQS 206_ VRPSL 211_ WKMML 216_ NIDVS 221_ ATAFY 226_ KAQPV 231_ IEFVC 236_ EVLDF 241_ KSIEE 246_ QQKPL 251_ TDSQR 256_ VKFTK 261_ EIKGL 266_ KVEIT 271_ HCGQM 276_ KRKYR 281_ VCNVT 286_ RRPAS 291_ HQTFP 296_ LQQES 301_ GQTVE 306_ CTVAQ 311_ YFKDR 316_ HKLVL 321_ RYPHL 326_ PCLQV 331_ GQEQK 336_ HTYLP 341_ LEVCN 346_ IVAGQ 351_ RCIKK 356_ LTDNQ 361_ TSTMI 366_ RATAR 371_ SAPDR 376_ QEEIS 381_ KLMRS 386_ ASFNT 391_ DPYVR 396_ EFGIM 401_ VKDEM 406_ TDVTG 411_ RVLQP 416_ PSILY 421_ GGRNK 426_ AIATP 431_ VQGVW 436_ DMRNK 441_ QFHTG 446_ IEIKV 451_ WAIAC 456_ FAPQR 461_ QCTEV 466_ HLKSF 471_ TEQLR 476_ KISRD 481_ AGMPI 486_ QGQPC 491_ FCKYA 496_ QGADS 501_ VEPMF 506_ RHLKN 511_ TYAGL 516_ QLVVV 521_ ILPGK 526_ TPVYA 531_ EVKRV 536_ GDTVL 541_ GMATQ 546_ CVQMK 551_ NVQRT 556_ TPQTL 561_ SNLCL 566_ KINVK 571_ LGGVN 576_ NILLP 581_ QGRPP 586_ VFQQP 591_ VIFLG 596_ ADVTH 601_ PPAGD 606_ GKKPS 611_ IAAVV 616_ GSMDA 621_ HPNRY 626_ CATVR 631_ VQQHR 636_ QEIIQ 641_ DLAAM 646_ VRELL 651_ IQFYK 656_ STRFK 661_ PTRII 666_ FYRDG 671_ VSEGQ 676_ FQQVL 681_ HHELL 686_ AIREA 691_ CIKLE 696_ KDYQP 701_ GITFI 706_ VVQKR 711_ HHTRL 716_ FCTDK 721_ NERVG 726_ KSGNI 731_ PAGTT 736_ VDTKI 741_ THPTE 746_ FDFYL 751_ CSHAG 756_ IQGTS 761_ RPSHY 766_ HVLWD 771_ DNRFS 776_ SDELQ 781_ ILTYQ 786_ LCHTY 791_ VRCTR 796_ SVSIP 801_ APAYY 806_ AHLVA 811_ FRARY 816_ HLVDK 821_ EHDSA 826_ EGSHT 831_ SGQSN 836_ GRDHQ 841_ ALAKA 846_ VQVHQ 851_DTLRT
1: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions
2: (Microbial infection) Upon Sars-CoV-2 infection, associates with viral miRNA-like small RNA, CoV2-miR-O7a, and may repress mRNAs, such as BATF2, to evade the IFN response