Lysosomal thioesterase PPT2 (PPT-2) (EC 3.1.2.-) (S-thioesterase G14)
1_MLGLC 6_ GQRLP 11_ AAWVL 16_ LLLPF 21_ LPLLL 26_ LAAPA 31_ PHRAS 36_ YKPVI 41_ VVHGL 46_ FDSSY 51_ SFRHL 56_ LEYIN 61_ ETHPG 66_ TVVTV 71_ LDLFD 76_ GRESL 81_ RPLWE 86_ QVQGF 91_ REAVV 96_ PIMAK 101_ APQGV 106_ HLICY 111_ SQGGL 116_ VCRAL 121_ LSVMD 126_ DHNVD 131_ SFISL 136_ SSPQM 141_ GQYGD 146_ TDYLK 151_ WLFPT 156_ SMRSN 161_ LYRIC 166_ YSPWG 171_ QEFSI 176_ CNYWH 181_ DPHHD 186_ DLYLN 191_ ASSFL 196_ ALING 201_ ERDHP 206_ NATVW 211_ RKNFL 216_ RVGHL 221_ VLIGG 226_ PDDGV 231_ ITPWQ 236_ SSFFG 241_ FYDAN 246_ ETVLE 251_ MEEQL 256_ VYLRD 261_ SFGLK 266_ TLLAR 271_ GAIVR 276_ CPMAG 281_ ISHTA 286_ WHSNR 291_ TLYET 296_CIEPW
1: Catalyzes the cleavage of thioester bonds from S-palmitoyl-CoA or S-palmitoyl-N-acetylcysteamine (unbranched structures) but does not have activity against palmitoylcysteine or palmitoylated proteins, branched structures or bulky head groups. Conversely, hydrolyzes both long and short chain fatty acyl-CoA substrate
2: Catalytically inactive due to lack of active site His-283